Summary | Transgene origin | Transgene description | Find Mice (IMSR) | References

Symbol:	Tg(Pf4-F5)1Dgi
Name:	transgene insertion 1, David Ginsburg
MGI ID:	MGI:3709380
Synonyms:	Pf4 Fv, Pf4fvA, Tg(Cxcl4-F5)1Dgi
Transgene:	Tg(Pf4-F5)1Dgi  Location: unknown
Alliance:	Tg(Pf4-F5)1Dgi page

Strain of Origin:

(C57BL/6 x SJL)F1 or C57BL/6 x (C57BL/6 x SJL)F1

Transgene Type:    Transgenic (Inserted expressed sequence) Mutation:    Insertion   Tg(Pf4-F5)1Dgi expresses 1 gene Transgene expresses: Organism Expressed Gene Note mouse F5 (MGI:88382)   Mutation details: The transgenic construct has the mouse Cxcl4 (Pf4, platelet factor 4) driving expression of the murine F5 (FV) gene including the entire coding sequence. An IRES-EGFP cassette with a bacterial gene was inserted after the F5 coding sequence. A chimeric intron with optimized splice signals was introduced upstream of the F5 cDNA enhance gene expression. Transgenic F5 expression is restricted to megakaryocyt-containing tissues (to spleen and bone marrow). EGFP expression is not detected. (J:86150)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:86150 Sun H, et al., The murine platelet and plasma factor V pools are biosynthetically distinct and sufficient for minimal hemostasis. Blood. 2003 Oct 15;102(8):2856-61
All:	3 reference(s)