Probe:	GCase cDNA1
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS7: epiblast	Trace
TS7: extraembryonic endoderm	Trace
TS7: trophectoderm	Trace
TS28: decidua	Weak			Expression was detected in decidual and sinusoidal cells.

Structure	Level	Pattern	Image	Note
TS16: embryo mesenchyme	Trace
TS16: nervous system	Trace
TS16: otocyst epithelium	Weak
TS16: telencephalon ventricular layer	Weak
TS16: lens pit	Weak			Expression was detected in the lens epithelial layer and all retinal layer cells.
TS16: optic cup	Weak			Expression was detected in the lens epithelial layer and all retinal layer cells.

Structure	Level	Pattern	Image	Note
TS20: embryo	Trace			Expression was detected in most cell types.
TS20: nervous system	Trace			Expression was detected in most cell types.
TS20: choroid invagination	Present
TS20: neural retinal epithelium	Present
TS20: pigmented retinal epithelium	Present
TS20: lens vesicle epithelium	Weak
TS20: liver	Weak
TS20: medulla oblongata	Weak
TS20: pituitary gland	Weak
TS20: trigeminal nerve	Weak
TS20: yolk sac	Weak
TS28: placenta	Weak
TS20: spinal cord	Weak	Regionally restricted		Expression was detected in the upper neural tube and ventral spinal cord cells.

Structure	Level	Pattern	Image	Note
TS22: embryo	Trace
TS22: nervous system	Trace
TS22: basal columns	Present
TS22: dorsal root ganglion	Present
TS22: spinal cord meninges	Present
TS22: cochlear duct epithelium	Weak
TS22: lens	Weak
TS22: otic capsule	Weak
TS22: retina	Present	Regionally restricted		Expression was weak in the retinal layers but was a moderate level in the ganglion cell layer.
TS22: epidermis	Present	Regionally restricted		Expression was detected in the outer epithelial cell layers of the developing epidermis.
TS22: skeleton	Present	Regionally restricted		Expression was detected in chondrocytes.
TS22: rib cartilage condensation	Weak
TS22: salivary gland	Weak	Regionally restricted		Expression was detected in epithelial cells.
TS22: intestine epithelium	Weak	Regionally restricted		Expression was weak in the intestinal villi epithelial cells.
TS22: metanephros	Weak	Regionally restricted		Expression was weak in the metanephros tubular epithelial cells.

Structure	Level	Pattern	Image	Note
TS24: embryo	Trace			Expression was detected in most cell types.
TS24: nervous system	Trace			Expression was detected in most cell types.
TS24: dorsal root ganglion	Present
TS24: spinal cord meninges	Present
TS24: ventral grey horn	Present
TS24: cochlear duct epithelium	Weak
TS24: lens	Weak
TS24: ear	Weak	Regionally restricted		Expression was detected in the otic capsule.
TS24: retina	Present	Regionally restricted		Expression was weak in the retinal layers but was a moderate level in the ganglion cell layer.
TS24: epidermis	Present	Regionally restricted		Expression was detected in the outer epithelial cell layers of the developing epidermis.
TS24: skeleton	Present	Regionally restricted		Expression was detected in the chondrocytes.
TS24: salivary gland	Weak	Regionally restricted		Expression was detected in the epithelial cells.
TS24: intestine epithelium	Weak	Regionally restricted		Expression was detected in the intestinal villi epithelial cells.
TS24: metanephros	Weak	Regionally restricted		Expression was detected in the metanephros tubular epithelial cells.

Structure	Level	Pattern	Image	Note
TS26: embryo	Trace			Expression was detected in most cell types.
TS26: brown fat	Present	Regionally restricted		Expression was detected in adipocytes.
TS26: heart	Weak	Regionally restricted		Expression was detected in cardiocytes.
TS26: skeletal muscle	Weak	Regionally restricted		Expression was detected in myocytes.
TS26: pancreas	Present	Regionally restricted		Expression was detected in acinar glands and islet epithelial cells.
TS26: intestine epithelium	Weak	Regionally restricted		Expression was weak in the intestinal villi epithelial cells.
TS26: brain	Weak
TS26: epidermis	Weak
TS26: skeleton	Weak	Regionally restricted		Expression was detected in the conversional zones of the developing bones.

Structure	Level	Pattern	Image	Note
TS8: embryo	Trace

Structure	Level	Pattern	Image	Note
TS12: yolk sac	Weak	Regionally restricted		Expression was detected in the yolk sac epithelium.
TS28: placenta	Weak	Regionally restricted		Expression was weak in the trophoblastic cells.

Structure	Level	Pattern	Image	Note
TS27: epidermis	Present	Regionally restricted	1A	Expression was detected in the outermost epidermal layers.

Structure	Level	Pattern	Image	Note
TS27: stomach	Strong	Regionally restricted	1B	Expression was detected in squamous cell mucosa of the forestomach.

Structure	Level	Pattern	Image	Note
TS27: small intestine	Strong	Regionally restricted	1C	Expression was detected in serosal cell layers and villi of the small intestine.

Structure	Level	Pattern	Image	Note
TS27: bone	Present	Regionally restricted	1D	Expression was detected in osteoblasts of bony trabeculae at the conversional zone.

Structure	Level	Pattern	Image	Note
TS27: small intestine	Strong	Regionally restricted	1F	Expression was detected in the mucosal epithelial cells of the small intestine. Moderate signal was detected at the serosal surface.
TS27: smooth muscle tissue	Absent		1F

Structure	Level	Pattern	Image	Note
TS27: small intestine	Strong	Regionally restricted	1G	Expression was detected in the mucosal epithelial cells of the small intestine. Moderate signal was detected at the serosal surface.
TS27: smooth muscle tissue	Absent		1G

Structure	Level	Pattern	Image	Note
TS27: pancreas	Strong		1H
TS27: seminiferous tubule epithelium	Strong		1H
TS27: metanephros	Strong	Regionally restricted	1H	Expression was detected in kidney tubular epithelial cells.
TS27: epididymis	Strong	Regionally restricted	1H	Expression was detected in tubules of the epididymus.
TS27: skeletal muscle	Weak		1H

Structure	Level	Pattern	Image	Note
TS27: cortical layer III	Present		1I
TS27: cortical layer V	Present		1I

Structure	Level	Pattern	Image	Note
TS27: cerebellum Purkinje cell layer	Strong		1J

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present		1K
TS27: hippocampus	Present		1K
TS27: meninges	Present		1K

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present		1L

Structure	Level	Pattern	Image	Note
TS28: epidermis	Present	Regionally restricted	1M	Expression was detected in the outermost epidermal layers.
TS28: hair follicle	Strong		1M

Structure	Level	Pattern	Image	Note
TS28: esophagus squamous epithelium	Strong		1N
TS28: smooth muscle tissue	Absent		1N

Structure	Level	Pattern	Image	Note
TS28: lymph node	Present	Regionally restricted	1O	Expression was detected in the sinusoidal capsular lining cells but no signal was detected in the lymphoid cell populations.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: Sertoli cell	Sertoli cell	Present		1P

Structure	Level	Pattern	Image	Note
TS27: cerebellum Purkinje cell layer	Strong
TS27: esophagus squamous epithelium	Strong
TS27: cerebellum	Present
TS27: cerebral cortex	Present
TS27: dorsal root ganglion	Present
TS27: hair follicle	Present
TS27: meninges	Present
TS27: retina ganglion cell layer	Present
TS27: spinal cord	Present
TS27: sweat gland	Present
TS27: bone	Strong	Regionally restricted		Expression was detected in the conversional zone cells.
TS27: stomach	Present	Regionally restricted		Expression was detected in the forestomach epithelial cells.
TS27: brain	Present	Regionally restricted		Expression was detected in the cerebral, cerebellar neurons and meningeal cells, but was weak in the glial cells.
TS27: photoreceptor layer	Present	Regionally restricted		Expression was detected in the rods and cones layers.
TS27: retina layer	Present	Regionally restricted		Expression was detected in the ganglion, rods and cones layers but was weak in other retinal cells.
TS27: skin	Strong	Regionally restricted		Expression was strong in mature epithelial cells of the skin.
TS27: liver	Weak	Uniform
TS27: lung	Weak	Uniform
TS27: intestine	Present	Regionally restricted		Expression was detected in the serosal cells and was strong in the mucosal epithelial cells.
TS27: islets of Langerhans	Strong	Regionally restricted		Expression was strong in the islet epithelial cells.
TS27: pancreatic acinus	Strong	Regionally restricted		Expression was strong in the acinar glands.
TS27: metanephros	Present	Regionally restricted		Expression was detected in the collecting tubular epithelial cells but was weak in other nephron epithelial cells.
TS27: seminiferous tubule epithelium	Weak
TS27: thymus	Strong	Regionally restricted		Expression was detected in thymocytes.
TS27: lymph node	Strong	Regionally restricted		Expression was strong in the intestinal lymph node capsular lining cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: hair follicle		Present
TS28: hippocampus		Present
TS28: sweat gland		Present
TS28: thalamus		Present	Regionally restricted		Expression was detected in the thalamic nuclei neurons.
TS28: adrenal gland		Weak
TS28: cerebellum Purkinje cell layer		Weak
TS28: epidermis		Strong	Regionally restricted		Expression was strong in the epithelial layer.
TS28: esophagus squamous epithelium		Strong
TS28: intestine		Present	Regionally restricted		Expression was detected in the mucosal and serosal cells.
TS28: pancreatic acinus		Strong			There is strong expression in the acinar cells.
TS28: metanephros		Weak	Regionally restricted		Expression was detected in the collecting tubular epithelial, juxtamedullary, and glomerular cells.
TS28: Sertoli cell	Sertoli cell	Weak
TS28: epithelium of ductus deferens		Weak			Expression was detected in the vas deferens epithelium.
TS28: lymph node		Present	Regionally restricted		Expression was detected in the capsular lining cells of the lymph node.
TS28: lung		Present	Uniform