Probe:	Aard cDNA3
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	The probe used in this assay detects both transcript isoforms.

Structure	Level	Pattern	Image	Note
TS21: testis	Strong		1B
TS21: embryo	Weak		1B	Whereas a strong signal is detected in the testis, only weak signals are detected in other tissues.

Structure	Level	Pattern	Image	Note
TS19: gonad	Ambiguous		2B XY 11.5	It was unclear whether expression levels were weak or absent.

Structure	Level	Pattern	Image	Note
TS20: testis	Present		2B XY 12.5	Expression is limited to the cord structures of the developing testis.

Structure	Level	Pattern	Image	Note
TS21: primary sex cord	Present		2B XY 13.5	Expression is limited to the cord structures of the developing testis.

Structure	Level	Pattern	Image	Note
TS23: testis	Present	Regionally restricted	2B XY 15.5	Expression is limited to the cord structures of the developing testis.

Structure	Level	Pattern	Image	Note
TS19: gonad	Ambiguous		2B XX 11.5	It was unclear whether expression levels were weak or absent.

Structure	Level	Pattern	Image	Note
TS20: ovary	Ambiguous		2B XX 12.5	It was unclear whether expression levels were weak or absent.

Structure	Level	Pattern	Image	Note
TS21: ovary	Ambiguous		2B XX 13.5	It was unclear whether expression levels were weak or absent.

Structure	Level	Pattern	Image	Note
TS23: ovary	Ambiguous		2B XX 15.5	It was unclear whether expression levels were weak or absent.

Structure	Level	Pattern	Image	Note
TS21: primary sex cord	Present		3B wt

Structure	Level	Pattern	Image	Note
TS21: primary sex cord	Present		3B we

Structure	Cell Type	Level	Pattern	Image	Note
TS21: Sertoli cell	Sertoli cell	Present		4B , 4E	Expression is localized to Sertoli cells. It is not detected in other cell types in the testis.
TS21: germ cell of testis		Absent		4E , 4B