Gene Expression Data
Assay Details
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Assay
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Reference:
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J:119269
Caqueret A, et al., Laminar organization of the early developing anterior hypothalamus. Dev Biol. 2006 Oct 1;298(1):95-106
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3704905
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Gene symbol:
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Pou3f2
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Gene name:
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POU domain, class 3, transcription factor 2
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Results
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Specimen
3J:
embryonic day 11.0
(more  )
Specimen
3J:
(close  )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: diencephalon |
Present |
Regionally restricted |
3J
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Expression was detected in the ventricular layer and extending into the mantle layer of the anterior hypothalamus.
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Specimen
3K:
embryonic day 11.0
(more )
Note: Double labeled: red - Pou3f2; green - Rgs4 (assay MGI:3703600). Expression domains were complementary.
Specimen
3K:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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Note:
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Double labeled: red - Pou3f2; green - Rgs4 (assay MGI:3703600). Expression domains were complementary.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: diencephalon |
Present |
Regionally restricted |
3K
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Expression was detected in the ventricular layer and extending into the mantle layer of the anterior hypothalamus.
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Specimen
4T:
embryonic day 11.5
(more )
Specimen
4T:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: diencephalon |
Present |
Regionally restricted |
4T
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Expression was detected in the anterior hypothalamus.
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Specimen
4U:
embryonic day 11.5
(more )
Note: Double labeled: red - Pou3f2; green - Rgs4 (assay MGI:3703600). Expression domains were complementary.
Specimen
4U:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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|
Note:
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Double labeled: red - Pou3f2; green - Rgs4 (assay MGI:3703600). Expression domains were complementary.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: diencephalon |
Present |
Regionally restricted |
4U
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Expression was detected in the anterior hypothalamus.
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Specimen
4W:
embryonic day 11.5
(more )
Specimen
4W:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: diencephalon |
Weak |
Regionally restricted |
4W
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Posteriorly, expression was decreased in the lateral aspect of the anterior hypothalamus but was present more medially.
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Specimen
4Y:
embryonic day 11.5
(more )
Note: Double labeled: red - Pou3f2; green - Rgs4 (assay MGI:3703600). Expression domains were complementary.
Specimen
4Y:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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Note:
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Double labeled: red - Pou3f2; green - Rgs4 (assay MGI:3703600). Expression domains were complementary.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: diencephalon |
Weak |
Regionally restricted |
4Y
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Posteriorly, expression was decreased in the lateral aspect of the anterior hypothalamus but was present more medially.
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Specimen
5T:
embryonic day 12.5
(more )
Specimen
5T:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hypothalamus mantle layer |
Present |
Regionally restricted |
5T
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Expression was not detected in the anterior aspect of the mantle layer of the anterior hypothalamus but was detected in the posterior aspect. Expression was restricted to the medial aspect of the neural tube.
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Specimen
5V:
embryonic day 12.5
(more )
Note: Double labeled: red - Pou3f2; green - Rgs4 (assay MGI:3703600). Expression domains were complementary.
Specimen
5V:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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|
Embedding:
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Paraffin
|
|
Note:
|
Double labeled: red - Pou3f2; green - Rgs4 (assay MGI:3703600). Expression domains were complementary.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hypothalamus mantle layer |
Present |
Regionally restricted |
5V
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Expression was not detected in the anterior aspect of the mantle layer of the anterior hypothalamus but was detected in the posterior aspect. Expression was restricted to the medial aspect of the neural tube.
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Specimen
5Z:
embryonic day 13.5
(more )
Specimen
5Z:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: hypothalamus mantle layer |
Present |
Regionally restricted |
5Z
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Expression was not detected in the anterior aspect of the mantle layer of the anterior hypothalamus but was detected in the posterior aspect. Expression was restricted to the medial aspect of the neural tube.
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Specimen
5AB:
embryonic day 13.5
(more )
Note: Double labeled: red - Pou3f2; green - Rgs4 (assay MGI:3703600). Expression domains were complementary.
Specimen
5AB:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
|
|
Embedding:
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Paraffin
|
|
Note:
|
Double labeled: red - Pou3f2; green - Rgs4 (assay MGI:3703600). Expression domains were complementary.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: hypothalamus mantle layer |
Present |
Regionally restricted |
5AB
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Expression was not detected in the anterior aspect of the mantle layer of the anterior hypothalamus but was detected in the posterior aspect. Expression was restricted to the medial aspect of the neural tube.
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Specimen
7C:
embryonic day 15.5
(more )
Specimen
7C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: hypothalamus |
Present |
Regionally restricted |
7C
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Expression was detected in a domain immediately adjacent to the third ventricle, which corresponds to the paraventricular nucleus.
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Specimen
7G:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Sim1tm1Jlmd/Sim1tm1Jlmd
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: hypothalamus |
Weak |
Regionally restricted |
7G
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Expression was reduced in the anterior hypothalamus of the mutant except in a subset of cells in a region corresponding to the lateral aspect of the paraventricular nucleus.
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Specimen
7M:
embryonic day 15.5
(more )
Specimen
7M:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: hypothalamus |
Present |
Regionally restricted |
7M
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Expression was detected in a domain immediately adjacent to the third ventricle, which corresponds to the paraventricular nucleus.
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Specimen
7Q:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Sim1tm1Jlmd/Sim1tm1Jlmd
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: hypothalamus |
Weak |
Regionally restricted |
7Q
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Expression was reduced in the anterior hypothalamus of the mutant except in a subset of cells in a region corresponding to the lateral aspect of the paraventricular nucleus.
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