Summary | Transgene origin | Transgene description | Find Mice (IMSR) | References

Symbol:	Tg(CAG-Bgeo,-AML1/ETO,-ALPP)1Lbe
Name:	transgene insertion 1, Corinne G Lobe
MGI ID:	MGI:3703145
Synonyms:	Tg(ACTB-Bgeo,-AML1/ETO,-ALPP)1Lbe, Tg(CAG-Bgeo,-AML1/ETO,-ALPP)1Lbe, ZAP-AML1-ETO
Transgene:	Tg(CAG-Bgeo,-AML1/ETO,-ALPP)1Lbe  Location: unknown
Alliance:	Tg(CAG-Bgeo,-AML1/ETO,-ALPP)1Lbe page

Germline Transmission:	Earliest citation of germline transmission: J:93700
Parent Cell Line:	R1 (ES Cell)
Strain of Origin:	(129X1/SvJ x 129S1/Sv)F1-Kitl+

Transgene Type:    Transgenic (Conditional ready, Humanized sequence, Inserted expressed sequence, Reporter) Mutation:    Insertion   Tg(CAG-Bgeo,-AML1/ETO,-ALPP)1Lbe expresses 1 gene Transgene expresses: Organism Expressed Gene Homolog in Mouse Note human ALPP (250) Alpi  (MGI:1924018)   Mutation details: A transgenic construct was designed with the CMV enhancer/chicken beta-actin promoter, loxP-flanked STOP sequence (beta-geo reporter followed by SV40 polyadenylation signal), human AML1-ETO fusion protein sequence (coding for the translocation t(8;21) present in 15% of acute myeloid leukemias (AML)), internal ribosomal entry site, and the human Placental Alkaline Phosphatase (ALPP or hPLAP) gene. Expression of lacZ is observed in all tissues including bone marrow progenitor cells. When bred to Cre recombinase expressing mice, the STOP sequence (and beta-geo) is removed in the resulting offspring, allowing transcription/co-expression of both the human AML1-ETO fusion protein and placental alkaline phosphatase (ALPP or PLAP) to proceed in the Cre-expressing cells. (J:119131)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:119131 Guo C, et al., Cre-conditional expression of the AML1-ETO fusion in transgenic mice. MGI Direct Data Submission. 2007;
All:	2 reference(s)