Pelp1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:100442 Pawlak J, et al., Developmental expression of MNAR mRNA in the mouse brain. Cell Tissue Res. 2005 Jun;320(3):545-9
Assay type:	RT-PCR
MGI Accession ID:	MGI:3700789
Gene symbol:	Pelp1
Gene name:	proline, glutamic acid and leucine rich protein 1

Probe:	MNAR-pA, MNAR-pB
Probe preparation:	labelled with SYBR green
Assay notes:	This data was obtained using real-time PCR. 5-8 individuals were pooled for each sample.

Results

Image: 2C

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	242 bp (b)	Amount	Genetic Background	Mutant Allele(s)	Sex
E15	E15.5 (a)	TS23: hypothalamus	Present	2 µg; total RNA	BALB/c		Male
E15	E15.5 (a)	TS23: hypothalamus	Present	2 µg; total RNA	BALB/c		Female
E17	E17.5 (a)	TS25: hypothalamus	Present	2 µg; total RNA	BALB/c		Male
E17	E17.5 (a)	TS25: hypothalamus	Present	2 µg; total RNA	BALB/c		Female
P0	P0	TS27: hypothalamus	Present	2 µg; total RNA	BALB/c		Male
P0	P0	TS27: hypothalamus	Present	2 µg; total RNA	BALB/c		Female
P7	P7	TS28: hypothalamus	Present	2 µg; total RNA	BALB/c		Male
P7	P7	TS28: hypothalamus	Present	2 µg; total RNA	BALB/c		Female
P15	P15	TS28: hypothalamus	Present	2 µg; total RNA	BALB/c		Male
P15	P15	TS28: hypothalamus	Weak	2 µg; total RNA	BALB/c		Female

Notes:
(a) Age normalized so that noon of plug day = E0.5.
(b) There is a stepwise decline of expression during pre- and postnatal development, reaching the lowest levels at P15. Highest expression levels are seen at E15. There is no observed difference in expression levels between the sexes.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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