Gene Expression Data
Assay Details
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Assay
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Reference:
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J:45780
Tiret L, et al., Increased apoptosis of motoneurons and altered somatotopic maps in the brachial spinal cord of Hoxc-8-deficient mice. Development. 1998 Jan;125(2):279-91
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3700202
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Gene symbol:
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Isl1
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Gene name:
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ISL1 transcription factor, LIM/homeodomain
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Antibody:
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anti-C-Isl-1 protein (K5)
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Detection system:
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Secondary antibody coupled to rhodamine
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Assay notes:
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Antibody used detects both Isl1 and Isl2; both genes have been annotated.
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Results
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Specimen
3A - C6:
embryonic day 13.5; Hoxc8tm1Phb/Hoxc8 +
(more  )
Note: Double labeled: red - Isl1 and Isl2; green - Hoxc8 (assay MGI:2149088).
Specimen
3A - C6:
(close  )
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Genetic Background:
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either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
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Mutant Allele(s):
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Hoxc8tm1Phb/Hoxc8+
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Isl1 and Isl2; green - Hoxc8 (assay MGI:2149088).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: spinal cord |
Present |
Single cells |
3A
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Expression was detected in motor neurons. The specimen was from the level of the cervical vertebra 6.
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Specimen
3B - C7:
embryonic day 13.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Note: Double labeled: red - Isl1 and Isl2; green - Hoxc8 (assay MGI:2149088).
Specimen
3B - C7:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
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Mutant Allele(s):
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Hoxc8tm1Phb/Hoxc8+
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Isl1 and Isl2; green - Hoxc8 (assay MGI:2149088).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: spinal cord |
Present |
Single cells |
3B
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Expression was detected in motor neurons. The specimen was from the level of the cervical vertebra 7.
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Specimen
3C - C8:
embryonic day 13.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Note: Double labeled: red - Isl1 and Isl2; green - Hoxc8 (assay MGI:2149088).
Specimen
3C - C8:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
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Mutant Allele(s):
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Hoxc8tm1Phb/Hoxc8+
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Isl1 and Isl2; green - Hoxc8 (assay MGI:2149088).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: spinal cord |
Present |
Single cells |
3C
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Expression was detected in motor neurons. The specimen was from the level of the cervical vertebra 8.
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Specimen
3D - T1:
embryonic day 13.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Note: Double labeled: red - Isl1 and Isl2; green - Hoxc8 (assay MGI:2149088).
Specimen
3D - T1:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
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Mutant Allele(s):
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Hoxc8tm1Phb/Hoxc8+
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Isl1 and Isl2; green - Hoxc8 (assay MGI:2149088).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: spinal cord |
Present |
Single cells |
3D
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Expression was detected in motor neurons. The specimen was from the level of the thoracic vertebra 1.
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Specimen
3E - C6:
embryonic day 14.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Note: Double labeled: red - Isl1 and Isl2; green - Hoxc8 (assay MGI:2149088).
Specimen
3E - C6:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
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Mutant Allele(s):
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Hoxc8tm1Phb/Hoxc8+
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Isl1 and Isl2; green - Hoxc8 (assay MGI:2149088).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: spinal cord |
Present |
Single cells |
3E
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Expression was detected in motor neurons. The specimen was from the level of the cervical vertebra 6.
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Specimen
3F - C7:
embryonic day 14.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Note: Double labeled: red - Isl1 and Isl2; green - Hoxc8 (assay MGI:2149088).
Specimen
3F - C7:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
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Mutant Allele(s):
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Hoxc8tm1Phb/Hoxc8+
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Isl1 and Isl2; green - Hoxc8 (assay MGI:2149088).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: spinal cord |
Present |
Single cells |
3F
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Expression was detected in motor neurons. The specimen was from the level of the cervical vertebra 7.
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Specimen
3G - C8 LMC:
embryonic day 14.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Note: Double labeled: red - Isl1 and Isl2; green - Hoxc8 (assay MGI:2149088).
Specimen
3G - C8 LMC:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
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Mutant Allele(s):
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Hoxc8tm1Phb/Hoxc8+
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Isl1 and Isl2; green - Hoxc8 (assay MGI:2149088).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: spinal cord |
Present |
Single cells |
3G
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Expression was detected in motor neurons in the lateral motor column. The specimen was from the level of the cervical vertebra 8.
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Specimen
3H - C8 MMC:
embryonic day 14.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Note: Double labeled: red - Isl1 and Isl2; green - Hoxc8 (assay MGI:2149088).
Specimen
3H - C8 MMC:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
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Mutant Allele(s):
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Hoxc8tm1Phb/Hoxc8+
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
|
Note:
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Double labeled: red - Isl1 and Isl2; green - Hoxc8 (assay MGI:2149088).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: spinal cord |
Present |
Single cells |
3H
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Expression was detected in motor neurons in the medial motor column. The specimen was from the level of the cervical vertebra 8.
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Specimen
4C:
embryonic day 12.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Note: Double labeled: red - Isl1 and Isl2; green - TUNEL.
Specimen
4C:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
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Mutant Allele(s):
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Hoxc8tm1Phb/Hoxc8+
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Isl1 and Isl2; green - TUNEL.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Present |
Single cells |
4C
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Expression was detected in motor neurons. The specimen was from the level of the cervical vertebra 8.
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Specimen
4D:
embryonic day 12.5; Hoxc8tm1Phb/ Hoxc8tm1Phb
(more )
Note: Double labeled: red - Isl1 and Isl2; green - TUNEL.
Specimen
4D:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
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Mutant Allele(s):
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Hoxc8tm1Phb/Hoxc8tm1Phb
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
|
Note:
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Double labeled: red - Isl1 and Isl2; green - TUNEL.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Present |
Single cells |
4D
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Expression was detected in motor neurons. The specimen was from the level of the cervical vertebra 8.
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Specimen
4G:
embryonic day 13.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Note: Double labeled: red - Isl1 and Isl2; green - TUNEL.
Specimen
4G:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
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Mutant Allele(s):
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Hoxc8tm1Phb/Hoxc8+
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Age:
|
embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
|
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
|
Note:
|
Double labeled: red - Isl1 and Isl2; green - TUNEL.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: spinal cord |
Present |
Single cells |
4G
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Expression was detected in motor neurons. The specimen was from the level of the cervical vertebra 8.
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Specimen
4H:
embryonic day 13.5; Hoxc8tm1Phb/ Hoxc8tm1Phb
(more )
Note: Double labeled: red - Isl1 and Isl2; green - TUNEL.
Specimen
4H:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
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Mutant Allele(s):
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Hoxc8tm1Phb/Hoxc8tm1Phb
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Age:
|
embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
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Cryosection
|
|
Note:
|
Double labeled: red - Isl1 and Isl2; green - TUNEL.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: spinal cord |
Present |
Single cells |
4H
|
Expression was detected in motor neurons. The specimen was from the level of the cervical vertebra 8.
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Specimen
4I:
embryonic day 13.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Specimen
4I:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
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Mutant Allele(s):
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Hoxc8tm1Phb/Hoxc8+
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Age:
|
embryonic day 13.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
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Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
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|
Embedding:
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Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: spinal cord |
Present |
Single cells |
4I
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Expression was detected in motor neurons.
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Specimen
4J:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
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Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8tm1Phb
|
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Age:
|
embryonic day 13.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: spinal cord |
Present |
Single cells |
4J
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Expression was detected in motor neurons, but there were fewer motor neurons in the mutant.
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Specimen
4K:
embryonic day 14.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Note: Double labeled: red - Isl1 and Isl2; green - TUNEL.
Specimen
4K:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
|
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Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8+
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: red - Isl1 and Isl2; green - TUNEL.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: spinal cord |
Present |
Single cells |
4K
|
Expression was detected in motor neurons. The specimen was from the level of the cervical vertebra 8.
|
Specimen
4L:
embryonic day 14.5; Hoxc8tm1Phb/ Hoxc8tm1Phb
(more )
Note: Double labeled: red - Isl1 and Isl2; green - TUNEL.
Specimen
4L:
(close )
|
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
|
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Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8tm1Phb
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: red - Isl1 and Isl2; green - TUNEL.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: spinal cord |
Present |
Single cells |
4L
|
Expression was detected in motor neurons, but there were fewer motor neurons in the mutant. The specimen was from the level of the cervical vertebra 8.
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Analysis Tools