Tg(KRT14-Esr1/HRAS)1Pkha Transgene Detail MGI Mouse (MGI:3700054)

Tg(KRT14-Esr1/HRAS)1Pkha
Transgene Detail

Summary

Symbol:	Tg(KRT14-Esr1/HRAS)1Pkha
Name:	transgene insertion 1, Paul A Khavari
MGI ID:	MGI:3700054
Synonyms:	K14-ER:Ras
Transgene:	Tg(KRT14-Esr1/HRAS)1Pkha Location: unknown
Alliance:	Tg(KRT14-Esr1/HRAS)1Pkha page

Transgene
origin

Strain of Origin:

C57BL/6

Transgene
description

Transgene Type:

Transgenic (Constitutively active, Humanized sequence, Inducible, Inserted expressed sequence)

Inducer:

tamoxifen

Mutation:

Insertion

Tg(KRT14-Esr1/HRAS)1Pkha expresses 1 gene

Mutation details: The transgene contains a 2075-bp human keratin 14 promoter construct driving expression of a cDNA that encodes a fusion protein comprising a constitutively active mutant form of the catalytic domain of human HRAS, in which glycine at amino acid position 12 is replaced by arginine (G12V), joined at its amino-terminal to a mutant mouse estrogen receptor ligand-binding domain in which replacement of glycine by arginine at amino acid position 525 (G525R, "ER^TM") renders the fusion protein's activity inducible by 4-hydroxytamoxifen, but unresponsive to natural estrogen (17beta-estradiol). (J:55801, J:81660, J:118583, J:118586, J:118587)

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Multiple mouse lines were generated, with varying transgene copy numbers and expression levels of the fusion protein. Lines were identified only as H, M, and L for high, medium and low copy number, respectively.

References

Original:	J:81660 Tarutani M, et al., Inducible activation of ras and raf in adult epidermis. Cancer Res. 2003 Jan 15;63(2):319-23
All:	10 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 04/16/2024 MGI 6.23