Reporter:	lacZ
	Direct Detection

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present	Regionally restricted	2A	The rostral limit of expression was at the level of the 8th pair of somites.
TS13: lateral plate mesoderm	Present	Regionally restricted	2A	The rostral limit of expression was at the level of the 13th pair of somites.
TS13: paraxial mesenchyme	Present	Regionally restricted	2A	The rostral limit of expression was at the level of the 13th pair of somites.
TS13: allantois	Present		2A

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present	Regionally restricted	2B	From the rostral region of the embryo to the posterior neuropore, expression was detected in the neurogenic ependymal layer.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	2C	The rostral limit of expression was at the level of the 8th pair of somites.
TS20: trunk mesenchyme	Present	Regionally restricted	2C	Expression was detected in paraxial mesoderm posteriorly to the first thoracic vertebra. The rostral limit of expression was at the level of the 13th pair of somites.
TS20: forelimb	Present	Regionally restricted	2C	Expression was detected in the rostral-half of the proximal forelimb bud.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Graded	2D	Expression was detected in a gradient in the ventral horn from C6 to C8-T1, in which the labeling reaches its highest level. Between 3rd to 5th cervical vertebrae, a small number of cells expressed b-gal at a low level in the intermediate zone.

Structure	Level	Pattern	Image	Note
TS21: basal columns	Present		2F	Expression was detected in the motor columns. The specimen was from the level of the eighth cervical vertebra (C8).

Structure	Level	Pattern	Image	Note
TS21: basal columns	Present		2G

Structure	Level	Pattern	Image	Note
TS21: basal columns	Present		2H	Expression was detected in cells, as expected, and their gross pattern of distribution along the mediolateral axis was not significantly modified.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Single cells	3I	The specimen was from the level of the cervical vertebra 6. At this level, expression was restricted to interneurons and/or glial cells.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Single cells	3J	The specimen was from the level of the cervical vertebra 7. At the rostral part of this level, expression was detected in a mosaic population of motor neurons. In the medial and caudal parts of this level, expression was in 100% of the motor neurons.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Single cells	3K	The specimen was from the level of the cervical vertebra 8. At this level, expression was detected in 100% of somatic motor neurons in the lateral motor column.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Single cells	3L	The specimen was from the level of the cervical vertebra 8. At this level, expression was detected in 100% of somatic motor neurons in the medial motor column.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Single cells	3M	The specimen was from the level of the cervical vertebra 6. At this level, expression was restricted to interneurons and/or glial cells.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Single cells	3N	The specimen was from the level of the cervical vertebra 7. At the rostral part of this level, expression was detected in a mosaic population of motor neurons.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Single cells	3O	The specimen was from the level of the cervical vertebra 8. At this level, expression was detected in somatic motor neurons (but not all) in the lateral motor column of the heterozygous mutant.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Single cells	3P	The specimen was from the level of the cervical vertebra 8. At this level, expression was detected in somatic motor neurons (but not all) in the medial motor column of the heterozygous mutant.