Probe:	mYaf2-a cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS16: embryo	Present		3B	Expression was distributed.
TS16: head	Strong	Regionally restricted	3B	Expression was detected in the prospective craniofacial region.
TS16: limb	Strong		3B
TS16: tail paraxial mesenchyme	Strong		3B
TS16: trunk paraxial mesenchyme	Strong		3B

Structure	Level	Pattern	Image	Note
TS16: head	Present		3D	Expression was detected in the cranial region.

Structure	Level	Pattern	Image	Note
TS16: nasal pit	Present	Regionally restricted	3E	Expression was detected in the olfactory placodes.
TS16: diencephalon lamina terminalis	Present		3E
TS16: future midbrain	Present	Regionally restricted	3E	Expression was detected in the dorsal half of the midbrain.
TS16: head mesenchyme	Present	Regionally restricted	3E	Expression was detected in the cephalic mesenchyme.

Structure	Level	Pattern	Image	Note
TS16: 1st branchial arch maxillary component	Present		3F
TS16: diencephalon lamina terminalis	Present		3F
TS16: future midbrain	Present	Regionally restricted	3F	Expression was detected in the dorsal half of the midbrain.
TS16: head mesenchyme	Present	Regionally restricted	3F	Expression was detected in the cephalic mesenchyme.

Structure	Level	Pattern	Image	Note
TS16: common atrial chamber	Present	Regionally restricted	3G	Expression was detected in the walls of the atrial chambers.
TS16: primitive ventricle cardiac muscle	Present	Regionally restricted	3G	Expression was detected in the myocardial wall of the ventricular chambers.
TS16: heart	Present	Regionally restricted	3G	Expression was detected in the wall of the atrial chambers and the myocardial wall of the ventricular chambers, but was weak in the tuberculation.

Structure	Level	Pattern	Image	Note
TS16: trunk dermomyotome	Present		3H	Expression levels were higher in the dermatome and myotome than in the sclerotome.
TS16: trunk myotome	Present		3H	Expression levels were higher in the dermatome and myotome than in the sclerotome.
TS16: trunk sclerotome	Present		3H	Expression levels were higher in the dermatome and myotome than in the sclerotome.
TS16: neural tube	Present	Graded	3H	Expression was detected in a dorso-ventral gradient.
TS16: migrating neural crest	Present		3I , 3H	Expression was detected in round-shaped migrating neural crest cells.

Structure	Level	Pattern	Image	Note
TS16: forelimb bud mesenchyme	Present	Regionally restricted	3J	Expression was detected in mesenchymal cells close to the surface ectoderm.

Structure	Level	Pattern	Image	Note
TS16: hindlimb bud mesenchyme	Present	Regionally restricted	3K	Expression was detected in mesenchymal cells close to the surface ectoderm.

Structure	Level	Pattern	Image	Note
TS16: tail dermomyotome	Present	Graded	3L	After de-epithelialization of the somitic mesoderm, expression was more intense in the dermomyotome than in the sclerotome.
TS16: tail sclerotome	Present	Graded	3L	After de-epithelialization of the somitic mesoderm, expression was more intense in the dermomyotome than in the sclerotome.

Structure	Level	Pattern	Image	Note
TS16: tail unsegmented mesenchyme	Present		3M	Expression was not graded in presomitic mesoderm.