Tg(Gad1-EGFP)98Agmo Transgene Detail MGI Mouse (MGI:3697311)

Tg(Gad1-EGFP)98Agmo
Transgene Detail

Summary

Symbol:	Tg(Gad1-EGFP)98Agmo
Name:	transgene insertion 98, Ariel Agmon
MGI ID:	MGI:3697311
Synonyms:	GAD67-GFP, Tg(Gad1/EGFP)98Agmo, X98
Transgene:	Tg(Gad1-EGFP)98Agmo Location: unknown
Alliance:	Tg(Gad1-EGFP)98Agmo page

Transgene
origin

Strain of Origin:

(C57BL/6 x SJL)F1

Transgene
description

Transgene Type:		Transgenic (Reporter)
Mutation:		Insertion
		Mutation details: The transgene comprises 10.3 kb of mouse genomic DNA containing the promoter region from the glutamic acid decarboxylase 1 (Gad1, commonly called GAD67) gene, including the 5' regulatory sequences, (noncoding) exon 1, intron 1 and a segment of exon 2, joined in-frame to the coding sequence of enhanced green fluorescent protein followed by an SV40 polyadenylation signal. The encoded fusion protein contains the first 17 amino acids of GAD1, 4 amino acids derived from the pEGFP-1 vector, and the entire enhanced green fluorescent protein. GFP expression is found in infragranular, calbindin-containing, layer 1-targeting cells that have propensity to fire low-threshold calcium spikes. (J:117601, J:117729)

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Of 11 initial lines derived from founders shown by PCR analysis to carry the transgene, 5 that expressed EGFP in the brain continued to be maintained. Two of these, designated by the authors X94 and X98, in which cortical EGFP expression was limited to nonpyramidal cells were subjected to further analysis.

References

Original:	J:117601 Ma Y, et al., Distinct subtypes of somatostatin-containing neocortical interneurons revealed in transgenic mice. J Neurosci. 2006 May 10;26(19):5069-82
All:	9 reference(s)

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