Itgb4 Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:48924 Murgia C, et al., Cell cycle and adhesion defects in mice carrying a targeted deletion of the integrin beta4 cytoplasmic domain. EMBO J. 1998 Jul 15;17(14):3940-51
Assay type:	Western blot
MGI Accession ID:	MGI:3694666
Gene symbol:	Itgb4
Gene name:	integrin beta 4

Antibody:	Anti-beta 4
Assay notes:	The protein was immunoprecipitated with an antibody to Itga6 (MGI:1342332) prior to loading.

Results

Image: 2B

	Sample Information		Bands				Other Sample Information
Lane	Age	Structure	Size Not Specified (a)	Size Not Specified (b)	Size Not Specified (c)	Size Not Specified (c)	Genetic Background	Mutant Allele(s)	Sex
+/+ C	Control
+/+ alpha6	E18.5 (d)	TS26: skin	Present (e)	Absent	Present (f)	Present (g)	involves: 129S1/Sv * C57BL/6		Not Specified
-/- C	Control
-/- alpha6	E18.5 (d)	TS26: skin	Absent	Present (e)	Absent	Absent	involves: 129S1/Sv * C57BL/6	Itgb4^tm1Fgg/Itgb4^tm1Fgg	Not Specified

Notes:
(a) Wild type band
(b) Tail-less
(c) This band represents a lower molecular weight form of wild type protein after proteolytic processing.
(d) Age of embryo at noon of plug day not specified in reference.
(e) The amount of the tail-less protein immunoprecipitated with Itga6 in the mutant was comparable with that of the wild type.
(f) Product A.
(g) Product B.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 04/23/2024 MGI 6.23