Tg(RP23-268L19-EGFP)2Mik
Transgene Detail
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| Symbol: |
Tg(RP23-268L19-EGFP)2Mik |
| Name: |
transgene insertion 2, Michael I Kotlikoff |
| MGI ID: |
MGI:3694555 |
| Synonyms: |
ChATBAC-eGFP, ChAT(BAC)-eGFP, ChAT line 2, Tg(RP23-268L19-eGFP)Kot |
| Transgene: |
Tg(RP23-268L19-EGFP)2Mik Location: unknown
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| Alliance: |
Tg(RP23-268L19-EGFP)2Mik page
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| Strain of Origin: |
Not Specified
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| Transgene Type: |
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Transgenic (Reporter) |
| Mutation: |
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Insertion
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Mutation details: The transgene consists of the bacterial artificial chromosome (BAC) clone 268L19 from the RP23 mouse genomic library, which contains the cholinesterase acetyltransferase (Chat) gene, in whose first intron resides the Sl18a3 (solute carrier family 18 (vesicular monoamine)) gene encoding the vesicular acetylcholine transporter (VAChT); ~78 kb of 5' and ~36 kb of 3' flanking DNA; and, inserted in place of the Chat translation initiation codon in exon 3, the enhanced green fluorescent protein (EGFP) gene followed by a polyadenylation signal. EGFP fluorescence is observed in cell bodies and processes of cholinergic neurons of both the central and peripheral nervous systems, including somatic motor and sensory neurons, parasympathetic neurons of various peripheral organ systems, and ganglionic neurons. Individual neuromuscular junctions of cardiac, skeletal and smooth muscle can be visualized. Individual gastrointestinal and respiratory mucosal epithelial cells and some associated lymphocytes also fluoresce.
(J:114554)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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Transgenic lines were derived from two transgenic founder mice and designated line 2 and line 44. No apparent differences were observed in their systemic patterns of EGFP expression either by fluorescent imaging or by immunohistochemical analysis.
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| Original: |
J:114554 Tallini YN, et al., BAC transgenic mice express enhanced green fluorescent protein in central and peripheral cholinergic neurons. Physiol Genomics. 2006 Nov 27;27(3):391-7 |
| All: |
59 reference(s) |
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Analysis Tools
