Gene Expression Data
Assay Details
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Assay
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Reference:
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J:100446
Okano S, et al., Examination of the Lunatic fringe and Uncx4.1 expression by whole-mount in situ hybridization in the embryo of the CKH-Jsr (jumbled spine and ribs) mouse. Jpn J Vet Res. 2005 Feb;52(4):145-9
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3694451
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Gene symbol:
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Uncx
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Gene name:
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UNC homeobox
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Probe:
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Uncx4.1 cDNA6
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2A:
embryonic day 9.5
(more  )
Specimen
2A:
(close  )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: trunk somite |
Present |
Regionally restricted |
2A
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Expression is restricted to the posterior halves of all somites.
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Specimen
2B:
embryonic day 9.5; Jsr/Jsr +
(more )
Specimen
2B:
(close )
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Genetic Background:
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involves: C57BL/6 * CKH
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Mutant Allele(s):
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Jsr/Jsr+
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: trunk somite |
Present |
Non-Uniform |
2B
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Unlike in wild-type, expression is observed in the whole somite. However, the expression pattern is mosaic and irregular with a generally lower level of expression than is seen in wild type.
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Analysis Tools