Gene Expression Data
Assay Details
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Assay
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Reference:
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J:80673
Bergo MO, et al., Defining the importance of phosphatidylserine synthase 2 in mice. J Biol Chem. 2002 Dec 6;277(49):47701-8
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:3693917
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Gene symbol:
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Ptdss2
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Gene name:
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phosphatidylserine synthase 2
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Reporter:
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lacZ
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Direct Detection
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Assay notes:
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The cryostat sections were fixed with 2% formaldehyde in phosphate-buffered saline supplemented with 0.2% glutaraldhyde.
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Results
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Specimen
6A:
(close  )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Ptdss2Gt(KST314)Byg/Ptdss2Gt(KST314)Byg
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Age:
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embryonic day 20.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Specimen
6C:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Ptdss2Gt(KST314)Byg/Ptdss2Gt(KST314)Byg
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Specimen
6E:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Ptdss2Gt(KST314)Byg/Ptdss2Gt(KST314)Byg
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hippocampus CA1 |
Present |
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6E
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Expression is present in the pyramidal neurons.
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Specimen
6F:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Ptdss2Gt(KST314)Byg/Ptdss2Gt(KST314)Byg
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Specimen
7A:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Ptdss2Gt(KST314)Byg/Ptdss2Gt(KST314)Byg
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Age:
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postnatal month 6
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Sex:
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Male
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Note:
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This animal has apparently normal looking testes.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: Sertoli cell |
Sertoli cell |
Strong |
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7A
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There is intense staining of the Sertoli cells of the spermatic ducts.
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| TS28: Leydig cell |
Leydig cell |
Present |
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7A
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There is punctate staining within the Leydig cells, but its intensity is less than that found in the Sertoli cells.
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| TS28: spermatocyte |
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Absent |
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7A
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No staining of spermatogonia or spermatocytes.
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| TS28: spermatogonium |
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Absent |
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7A
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No staining of spermatogonia or spermatocytes.
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Specimen
7B:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Ptdss2Gt(KST314)Byg/Ptdss2Gt(KST314)Byg
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Age:
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postnatal month 6
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Sex:
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Male
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Note:
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This animal has atrophic testes.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: Sertoli cell |
Sertoli cell |
Strong |
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7B
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There is intense staining of the Sertoli cells even though the spermatic ducts of this animal are devoid of spermatagonia.
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)
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