Gene Expression Data
Assay Details
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Assay
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Reference:
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J:54261
Ward PP, et al., Restricted spatiotemporal expression of lactoferrin during murine embryonic development. Endocrinology. 1999 Apr;140(4):1852-60
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3693838
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Gene symbol:
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Ltf
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Gene name:
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lactotransferrin
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Antibody:
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anti-lactoferrin
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Detection system:
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Secondary antibody coupled to fluorescein
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Results
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Specimen
2A:
embryonic day 2.5
(more  )
Note: 8-cell stage.
Specimen
2A:
(close  )
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Genetic Background:
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either: (involves: 129 * C57BL/6) or (involves: 129 * C57BL/6 * ICR)
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Age:
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embryonic day 2.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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8-cell stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS3: 8-cell stage embryo |
Present |
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2A
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Expression is located extracellularly in the perivitelline space separating the blastomeres from the zona pellucida.
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Specimen
2B:
embryonic day 3.0
(more )
Note: 16-cell stage.
Specimen
2B:
(close )
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Genetic Background:
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either: (involves: 129 * C57BL/6) or (involves: 129 * C57BL/6 * ICR)
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Age:
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embryonic day 3.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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16-cell stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: compacted morula |
Present |
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2B
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Expression is selectively localized within the cells of the outer trophectodermal lineage, which is a pattern distinctly different from that observed for the mRNA.
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Specimen
2C:
embryonic day 3.5
(more )
Note: 32-cell stage.
Specimen
2C:
(close )
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Genetic Background:
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either: (involves: 129 * C57BL/6) or (involves: 129 * C57BL/6 * ICR)
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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32-cell stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: compacted morula |
Present |
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2C
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Expression is selectively localized within the cells of the outer trophectodermal lineage, which is a pattern distinctly different from that observed for the mRNA.
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Analysis Tools