Probe:	RIKEN clone 6230400M02
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS8: anterior visceral endoderm	Present		1A

Structure	Level	Pattern	Image	Note
TS10: anterior visceral endoderm	Present	Regionally restricted	1B	Expression is in the migrating anterior visceral endoderm.
TS10: primitive streak	Weak	Regionally restricted	1B	Expression is in the mesoderm cells arising from the anterior primitive streak.

Structure	Level	Pattern	Image	Note
TS10: anterior visceral endoderm	Present		1C

Structure	Level	Pattern	Image	Note
TS11: definitive endoderm	Present	Regionally restricted	1D	Expression is in the definitive endoderm cells underlying the prospective head-fold.

Structure	Level	Pattern	Image	Note
TS10: definitive endoderm	Present	Regionally restricted	2A	Expression is in the rostral anterior definitive endoderm and is absent in the midline.

Structure	Level	Pattern	Image	Note
TS11: definitive endoderm	Present		2B
TS11: embryo mesoderm	Present	Regionally restricted	2B	Expression is in the intra-embryonic mesoderm subjacent to the caudal part of the headfold.
TS11: mesenchyme derived from head mesoderm	Absent		2B
TS11: neural ectoderm	Absent		2B
TS11: notochordal plate	Absent		2B

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present	Regionally restricted	2B1	Expression is in the intra-embryonic mesoderm subjacent to the caudal part of the headfold.
TS11: yolk sac mesoderm	Present		2B1

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present	Regionally restricted	2B2	Expression is in the intra-embryonic mesoderm subjacent to the caudal part of the headfold.

Structure	Level	Pattern	Image	Note
TS12: embryo endoderm	Present		2C
TS12: foregut diverticulum	Present		2C

Structure	Level	Pattern	Image	Note
TS12: foregut diverticulum endoderm	Strong		2C1
TS12: midgut	Strong		2C1

Structure	Level	Pattern	Image	Note
TS12: foregut diverticulum endoderm	Strong		2C2
TS12: midgut	Strong		2C2

Structure	Level	Pattern	Image	Note
TS12: foregut diverticulum endoderm	Strong		2C3
TS12: midgut	Strong		2C3

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon neural fold	Present		2D
TS13: vitelline vein	Present		2D
TS13: embryo	Present	Regionally restricted	2D	Expression is in the body wall overlying the pericardial cavity.

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon neural fold	Present	Graded	2D1	Expression is stronger in the dorsal aspects of the neural folds.

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon neural fold	Present	Graded	2D2	Expression is stronger in the dorsal aspects of the neural folds.

Structure	Level	Pattern	Image	Note
TS13: foregut diverticulum	Present	Regionally restricted	2D3	Expression is in the ventral endoderm foregut diverticulum specifically in the buccopharyngeal membrane.

Structure	Level	Pattern	Image	Note
TS13: vitelline vein	Present		2D4
TS13: embryo	Present	Regionally restricted	2D4	Expression is in the body wall overlying the pericardial cavity.

Structure	Level	Pattern	Image	Note
TS13: embryo	Present	Regionally restricted	2D5	In the region where the neural tube is closed, expression is visible in the endoderm continuous with the yolk sac.
TS13: yolk sac endoderm	Present	Regionally restricted	2D5	In the region where the neural tube is closed, expression is visible in the endoderm continuous with the yolk sac.

Structure	Level	Pattern	Image	Note
TS13: hindgut diverticulum endoderm	Strong	Regionally restricted	2D6	Expression is in the ventral endoderm of hindgut diverticulum.
TS13: vitelline artery	Present		2D6
TS13: embryo	Present	Regionally restricted	2D6	Expression is in the endoderm continuous with the yolk sac.
TS13: yolk sac	Present	Regionally restricted	2D6	Expression is in the endoderm continuous with the yolk sac.

Structure	Level	Pattern	Image	Note
TS14: prosencephalon	Present		2E
TS14: trunk	Present	Regionally restricted	2E	Expression is in a continuous domain that extends along the ventral region of the trunk.

Structure	Level	Pattern	Image	Note
TS14: prosencephalon	Present		2E1	Expression is dorsal in the neural folds.

Structure	Level	Pattern	Image	Note
TS14: 1st branchial arch mandibular component ectoderm	Present		2E2

Structure	Level	Pattern	Image	Note
TS14: trunk somite	Weak	Regionally restricted	2E3	Expression is in the somites at the heart region.

Structure	Level	Pattern	Image	Note
TS14: endocardial tube	Present	Regionally restricted	2E4	Expression isin the endocardial cells adjacent to the septum transversum.

Structure	Level	Pattern	Image	Note
TS14: trunk somite	Weak	Regionally restricted	2E5	Expression is in the somites in the tail region.

Structure	Level	Pattern	Image	Note
TS14: hindgut diverticulum	Present		2E6
TS14: mesenchyme derived from splanchnopleure	Present		2E6
TS14: midgut	Present		2E6
TS14: vitelline artery	Present		2E6
TS14: embryo	Present	Regionally restricted	2E6	Expression is in the endoderm in contact with the somatopleure layer.

Structure	Level	Pattern	Image	Note
TS14: branchial arch	Present		2F
TS14: future diencephalon roof plate	Present		2F
TS14: mesenchyme derived from splanchnopleure	Present		2F
TS14: midgut	Present		2F
TS14: vitelline artery	Present		2F
TS14: embryo	Present	Regionally restricted	2F	Expression is in the endoderm cells in contact with the somatopleure layer.
TS14: 1st branchial arch	Present	Regionally restricted	2F	Expression is in the epithelium.

Structure	Level	Pattern	Image	Note
TS14: branchial arch	Present		2F1	Expression is in the endoderm cells in contact with the somatopleure layer.
TS14: mesenchyme derived from splanchnopleure	Present		2F1	Expression is in the endoderm cells in contact with the somatopleure layer.
TS14: midgut	Present		2F1	Expression is in the endoderm cells in contact with the somatopleure layer.
TS14: vitelline artery	Present		2F1	Expression is in the endoderm cells in contact with the somatopleure layer.
TS14: 1st branchial arch	Present	Regionally restricted	2F1	Expression is in the epithelium.
TS14: future diencephalon roof plate	Present		2F1

Structure	Level	Pattern	Image	Note
TS14: branchial arch	Present		2F2
TS14: mesenchyme derived from splanchnopleure	Present		2F2
TS14: midgut	Present		2F2
TS14: vitelline artery	Present		2F2
TS14: embryo	Present	Regionally restricted	2F2	Expression is in the endoderm cells in contact with the somatopleure layer.

Structure	Level	Pattern	Image	Note
TS14: branchial arch	Present		2F3
TS14: mesenchyme derived from splanchnopleure	Present		2F3
TS14: midgut	Present		2F3
TS14: vitelline artery	Present		2F3
TS14: embryo	Present	Regionally restricted	2F3	Expression is in the endoderm cells in contact with the somatopleure layer.