Crhr1 RT-PCR Gene Expression Assay - GXD

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Gene Expression Data
Assay Details

Assay

Reference:	J:96061 Juriloff DM, et al., Investigations of the genomic region that contains the clf1 mutation, a causal gene in multifactorial cleft lip and palate in mice. Birth Defects Res A Clin Mol Teratol. 2005 Feb;73(2):103-13
Assay type:	RT-PCR
MGI Accession ID:	MGI:3689031
Gene symbol:	Crhr1
Gene name:	corticotropin releasing hormone receptor 1

Probe:	Crhr1-pC, Crhr1-pD
Visualized with:	Ethidium bromide
Assay notes:	A set of nested primers was used to amplify this gene. See MGI:3688910 for the first round primers.

Results

Image: 2_Crhr1

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	315 bp (a)	Amount	Genetic Background	Mutant Allele(s)	Sex
A Lane 1	E10.0-11.0	TS17: head	Present	Not Specified; total RNA	A/WySnJ-ctl/GrsrJ	ctl/ctl	Not Specified
A Lane 2	E10.0-11.0	TS17: head	Present	Not Specified; total RNA	A/WySnJ-ctl/GrsrJ	ctl/ctl	Not Specified
B Lane 1	E10.0-11.0	TS17: head	Present	Not Specified; total RNA	AXB4/PgnJ		Not Specified
B Lane 2	E10.0-11.0	TS17: head	Present	Not Specified; total RNA	AXB4/PgnJ		Not Specified
C Lane 1	Control
C Lane 2	Control
D Lane 1	Control
D Lane 2	Control
E Lane 1	P adult	TS28: testis	Present	Not Specified; total RNA	A/WySnJ-ctl/GrsrJ	ctl/ctl	Male
E Lane 2	P adult	TS28: testis	Present	Not Specified; total RNA	A/WySnJ-ctl/GrsrJ	ctl/ctl	Male

Notes:
(a) No difference in product size or quantity was noted between the strains.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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