Probe:	Rnf2 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex ventricular layer	Strong		1B
TS21: 3rd ventricle	Present		1B
TS21: cerebellum	Present		1B
TS21: thalamus	Present		1B
TS21: midbrain	Present	Regionally restricted	1B	Expression is in the tegmentum.
TS21: midbrain roof plate	Present	Regionally restricted	1B	Expression is in the tectum.

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present		1F	Expression is in both germinal zones of the anterior telencephalon: the dorsal and medial neuroepithelium of the pallium, as well as the ventral basal ganglia.

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present	Regionally restricted	1G	Expression is in the posterior telencephalic neuroepithelia.
TS21: forebrain	Present	Regionally restricted	1G	Expression is in the developing hippocampus.
TS21: 3rd ventricle	Present		1G
TS21: thalamus	Present		1G
TS21: hypothalamus	Present	Regionally restricted	1G	Expression is in a subset of cells.

Structure	Level	Pattern	Image	Note
TS21: cortical plate	Weak	Regionally restricted	1K	xpression is in post-mitotic cells of the cortical plate.

Structure	Level	Pattern	Image	Note
TS21: forebrain	Present	Regionally restricted	1N	Expression is in the developing hippocampus.

Structure	Level	Pattern	Image	Note
TS21: cerebellum	Present		1Q
TS21: midbrain	Strong	Regionally restricted	1Q	Expression is in the inferior colliculus.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex ventricular zone	Present		4B , 4B" , 4B'
TS26: telencephalon	Present	Regionally restricted	4B" , 4B' , 4B	Expression is in the basal ganglia.
TS26: cortical plate	Weak		4B" , 4B' , 4B

Structure	Level	Pattern	Image	Note
TS17: brain	Absent

Structure	Level	Pattern	Image	Note
TS26: hippocampus	Present	Regionally restricted	5B	Expression is in CA1-3. Higher magnification was shown in the inset.
TS26: dentate gyrus	Present		5B	Higher magnification was shown in the inset.

Structure	Level	Pattern	Image	Note
TS26: main olfactory bulb	Present	Regionally restricted	6C	Higher magnification was shown in the inset. Expression is in neuroepithelial and granular cells in the ventricular zone and the granular layer of the bulbus.

Structure	Level	Pattern	Image	Note
TS26: cerebellum	Present	Regionally restricted	7H' , 7H	Expression is in the purkinje cell layer and the external granule cell layer and is absent in the molecular layer.
TS26: midbrain	Present	Regionally restricted	7H' , 7H	Expression is in the superior and inferior colliculi.

Structure	Level	Pattern	Image	Note
TS26: rostral migratory stream	Present		8C

Structure	Level	Pattern	Image	Note
TS26: rostral migratory stream	Present		8F

Structure	Level	Pattern	Image	Note
TS27: rostral migratory stream	Present