Gene Expression Data
Assay Details
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Assay
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Reference:
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J:80666
Smits P, et al., The transcription factors L-Sox5 and Sox6 are essential for cartilage formation. Dev Cell. 2001 Aug;1(2):277-90
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3687130
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Gene symbol:
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Col2a1
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Gene name:
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collagen, type II, alpha 1
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Probe:
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pMCol2a1-1
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Probe preparation:
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Antisense labelled with S35
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Results
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Specimen
4B left:
embryonic day 12.5
(more  )
Specimen
4B left:
(close  )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Specimen
4B right:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Sox5tm1Vlf/Sox5tm1Vlf
Sox6tm1Vlf/Sox6tm1Vlf
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Specimen
4C Col2a1:
embryonic day 12.5
(more )
Specimen
4C Col2a1:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: limb pre-cartilage condensation |
Present |
Regionally restricted |
4C Col2a1
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Expression was weak in the most distal precartilaginous condensations of hindlimb buds but was high throughout the more proximal precartilaginous condensations of hindlimb buds.
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Specimen
6A Collagen 2 left:
embryonic day 15.5; Sox5tm1Vlf/Sox5 +
(more )
Specimen
6A Collagen 2 left:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Sox5tm1Vlf/Sox5+
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Specimen
6A Collagen 2 right:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Sox5tm1Vlf/Sox5tm1Vlf
Sox6tm1Vlf/Sox6tm1Vlf
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Analysis Tools