Probe:	Grhl3 cDNA2
Probe preparation:	Antisense labelled with P33 RNA

Structure	Level	Pattern	Image	Note
TS13: surface ectoderm	Strong	Regionally restricted	1C	Expression was detected in a small cohort of non-neural ectoderm cells, located immediately adjacent to the folding neural plate.

Structure	Level	Pattern	Image	Note
TS17: surface ectoderm	Present	Uniform	1F

Structure	Level	Pattern	Image	Note
TS20: skin	Present	Uniform	1I

Structure	Level	Pattern	Image	Note
TS23: skin	Present	Uniform	1L	Expression was detected in all the supra-basal layers.

Structure	Level	Pattern	Image	Note
TS25: skin	Present	Uniform	1O	Expression was detected in all the supra-basal layers.

Structure	Level	Pattern	Image	Note
TS24: skin	Present	Uniform	1R	Expression was detected in all the supra-basal layers.

Structure	Level	Pattern	Image	Note
TS13: embryo	Present	Regionally restricted		Expression was detected in surface ectoderm but was absent from: submandibular/sublingual glands, heart, lung, kidney, olfactory epithelium, oral epithelium, oesophagus, stomach, midgut, hindgut and urogenital tract.
TS13: surface ectoderm	Strong
TS13: oral region	Absent			Expression was absent from oral epithelium.
TS13: genitourinary system	Absent
TS13: heart	Absent
TS13: hindgut diverticulum	Absent
TS13: midgut	Absent

Structure	Level	Pattern	Image	Note
TS17: embryo	Present	Regionally restricted		Expression was detected in surface ectoderm, heart, oral epithelium, oesophagus, hindgut and urogenital tract but was absent from: submandibular/sublingual glands, lung, kidney, olfactory epithelium, stomach and midgut.
TS17: heart	Strong
TS17: esophagus	Present
TS17: genitourinary system	Present
TS17: hindgut	Present
TS17: oral epithelium	Present
TS17: surface ectoderm	Present
TS17: lung	Absent
TS17: midgut	Absent
TS17: olfactory epithelium	Absent
TS17: stomach	Absent
TS17: oral region gland	Absent			Expression was absent from submandibular/sublingual glands.

Structure	Level	Pattern	Image	Note
TS20: esophagus	Present
TS20: heart	Present
TS20: hindgut	Present
TS20: oral epithelium	Present
TS20: skin	Present
TS20: urinary system	Present			Expression was detected in urogenital tract/bladder.
TS20: lung	Absent
TS20: metanephros	Absent
TS20: midgut	Absent
TS20: olfactory epithelium	Absent
TS20: stomach	Absent
TS20: gland	Absent			Expression was absent from the submandibular/sublingual glands.

Structure	Level	Pattern	Image	Note
TS23: oral epithelium	Strong
TS23: stomach fundus	Strong
TS23: bladder	Strong			Expression was strong in the urogenital tract/bladder.
TS23: reproductive system	Strong			Expression was strong in the urogenital tract/bladder.
TS23: esophagus	Present
TS23: hindgut	Present
TS23: skin	Present
TS23: heart	Weak
TS23: lung	Weak
TS23: metanephros	Absent
TS23: midgut	Absent
TS23: olfactory epithelium	Absent
TS23: stomach glandular region	Absent
TS23: sublingual gland primordium	Absent
TS23: submandibular gland primordium	Absent

Structure	Level	Pattern	Image	Note
TS25: oral epithelium	Strong
TS25: stomach fundus	Strong
TS25: bladder	Strong			Expression was strong in the urogenital tract/bladder.
TS25: reproductive system	Strong			Expression was strong in the urogenital tract/bladder.
TS25: esophagus	Present
TS25: skin	Present
TS25: anal region	Present			Expression was detected in the hindgut/large intestine.
TS25: colon	Present			Expression was detected in the hindgut/large intestine.
TS25: rectum	Present			Expression was detected in the hindgut/large intestine.
TS25: metanephros	Weak
TS25: heart	Absent
TS25: lung	Absent
TS25: olfactory epithelium	Absent
TS25: stomach glandular region	Absent
TS25: sublingual gland	Absent
TS25: submandibular gland	Absent
TS25: duodenum	Absent			Expression was absent from midgut/small intestine.
TS25: ileum	Absent			Expression was absent from midgut/small intestine.
TS25: jejunum	Absent			Expression was absent from midgut/small intestine.

Structure	Level	Pattern	Image	Note
TS23: olfactory epithelium	Absent		2C

Structure	Level	Pattern	Image	Note
TS24: oral epithelium	Strong		2F

Structure	Level	Pattern	Image	Note
TS23: esophagus	Present		2I

Structure	Level	Pattern	Image	Note
TS25: stomach fundus	Strong		2L
TS25: stomach glandular region	Absent		2L

Structure	Level	Pattern	Image	Note
TS24: anal canal	Strong		2O
TS24: colon	Present		2O	Expression was detected in distal hindgut.
TS24: bladder	Present	Regionally restricted	2O	Expression was detected in the epithelial lining.
TS24: ureter	Present	Regionally restricted	2O	Expression was detected in the epithelial lining.
TS24: urethra of male	Present	Regionally restricted	2O	Expression was detected in the epithelial lining.

Structure	Level	Pattern	Image	Note
TS17: endocardial cushion	Present		3G
TS17: outflow tract	Present		3G

Structure	Level	Pattern	Image	Note
TS20: endocardial cushion	Present		3H
TS20: outflow tract	Present		3H

Structure	Level	Pattern	Image	Note
TS23: cardiac muscle tissue	Weak		3I	Expression was detected at lower levels in the myocardium.

Structure	Level	Pattern	Image	Note
TS20: trachea	Weak	Regionally restricted	4C	Expression was confined to the lining of the trachea and larger airways.

Structure	Level	Pattern	Image	Note
TS22: trachea	Weak	Regionally restricted	4F	Expression was confined to the lining of the trachea and larger airways.

Structure	Level	Pattern	Image	Note
TS23: trachea	Weak	Regionally restricted	4I	Expression was confined to the lining of the trachea and larger airways.

Structure	Level	Pattern	Image	Note
TS24: trachea	Weak	Regionally restricted	4L	Expression was confined to the lining of the trachea and larger airways.

Structure	Level	Pattern	Image	Note
TS25: trachea	Weak	Regionally restricted	4O	Expression was confined to the lining of the trachea and larger airways.

Structure	Level	Pattern	Image	Note
TS22: metanephros	Present	Regionally restricted	5C	Expression was largely confined to the maturing epithelial portions of the kidney.

Structure	Level	Pattern	Image	Note
TS23: renal pelvis	Present		5F

Structure	Level	Pattern	Image	Note
TS24: metanephros	Present		5I

Structure	Level	Pattern	Image	Note
TS25: metanephros	Present		5L