Probe:	Grhl1 cDNA1
Probe preparation:	Antisense labelled with P33 RNA

Structure	Level	Pattern	Image	Note
TS13: surface ectoderm	Absent		1A

Structure	Level	Pattern	Image	Note
TS17: surface ectoderm	Weak		1D

Structure	Level	Pattern	Image	Note
TS20: skin	Weak		1G

Structure	Level	Pattern	Image	Note
TS23: skin	Strong		1J

Structure	Level	Pattern	Image	Note
TS25: skin	Strong		1M

Structure	Level	Pattern	Image	Note
TS24: skin	Strong	Uniform	1P	Expression was detected in all suprabasal levels.

Structure	Level	Pattern	Image	Note
TS13: embryo	Present	Regionally restricted		Expression was detected in the heart but was absent from: surface ectoderm, submandibular/sublingual glands, lung, kidney, olfactory epithelium, oral epithelium, oesophagus/foregut, stomach, midgut, hindgut, urogenital tract/bladder.
TS13: heart	Present
TS13: oral region	Absent			Expression was absent from the oral epithelium.
TS13: hindgut diverticulum	Absent
TS13: midgut	Absent
TS13: surface ectoderm	Absent
TS13: genitourinary system	Absent			Expression was absent from urogenital tract/bladder.

Structure	Level	Pattern	Image	Note
TS17: embryo	Present	Regionally restricted		Expression was detected in surface ectoderm and heart but was absent from submandibular/sublingual glands, lung, kidney, olfactory epithelium, oral epithelium, oesophagus, stomach, midgut, hindgut, urogenital tract/bladder.
TS17: heart	Strong
TS17: surface ectoderm	Weak
TS17: esophagus	Absent
TS17: hindgut	Absent
TS17: lung	Absent
TS17: midgut	Absent
TS17: olfactory epithelium	Absent
TS17: oral epithelium	Absent
TS17: stomach	Absent
TS17: oral region gland	Absent			Expression was absent from submandibular/sublingual glands.
TS17: genitourinary system	Absent			Expression was absent from urogenital tract/bladder.

Structure	Level	Pattern	Image	Note
TS20: heart	Strong
TS20: olfactory epithelium	Strong
TS20: skin	Weak
TS20: esophagus	Absent
TS20: hindgut	Absent
TS20: lung	Absent
TS20: metanephros	Absent
TS20: midgut	Absent
TS20: oral epithelium	Absent
TS20: stomach	Absent
TS20: gland	Absent			Expression was absent from submandibular/sublingual glands.
TS20: urinary system	Absent			Expression was absent from urogenital tract/bladder.

Structure	Level	Pattern	Image	Note
TS23: bladder	Strong
TS23: heart	Strong
TS23: olfactory epithelium	Strong
TS23: oral epithelium	Strong
TS23: skin	Strong
TS23: stomach fundus	Strong
TS23: reproductive system	Strong			Expression was strong in the urogenital tract.
TS23: esophagus	Present
TS23: lung	Present
TS23: metanephros	Present
TS23: sublingual gland primordium	Present
TS23: submandibular gland primordium	Present
TS23: hindgut	Absent
TS23: midgut	Absent
TS23: stomach glandular region	Absent

Structure	Level	Pattern	Image	Note
TS25: bladder	Strong
TS25: olfactory epithelium	Strong
TS25: oral epithelium	Strong
TS25: skin	Strong
TS25: stomach fundus	Strong
TS25: sublingual gland	Strong
TS25: submandibular gland	Strong
TS25: reproductive system	Strong			Expression was strong in the urogenital tract.
TS25: esophagus	Present
TS25: heart	Present
TS25: lung	Weak
TS25: metanephros	Weak
TS25: stomach glandular region	Absent
TS25: duodenum	Absent			Expression was absent from midgut/small intestine.
TS25: ileum	Absent			Expression was absent from midgut/small intestine.
TS25: jejunum	Absent			Expression was absent from midgut/small intestine.
TS25: anal region	Absent			Expression was absent from hindgut/large intestine.
TS25: colon	Absent			Expression was absent from hindgut/large intestine.
TS25: rectum	Absent			Expression was absent from hindgut/large intestine.

Structure	Level	Pattern	Image	Note
TS23: olfactory epithelium	Strong		2A

Structure	Level	Pattern	Image	Note
TS24: oral epithelium	Strong		2D

Structure	Level	Pattern	Image	Note
TS23: esophagus	Present		2G

Structure	Level	Pattern	Image	Note
TS25: stomach fundus	Strong		2J
TS25: stomach glandular region	Absent		2J

Structure	Level	Pattern	Image	Note
TS24: anal canal	Strong		2M
TS24: bladder	Present	Regionally restricted	2M	Expression was detected in the epithelial lining.
TS24: ureter	Present	Regionally restricted	2M	Expression was detected in the epithelial lining.
TS24: urethra of male	Present	Regionally restricted	2M	Expression was detected in the epithelial lining.
TS24: colon	Absent		2M	Expression was absent from distal hindgut.

Structure	Level	Pattern	Image	Note
TS13: endocardial tube	Present		3A	Expression was detected in the endocardial lining of the heart tube.

Structure	Level	Pattern	Image	Note
TS17: atrioventricular canal	Strong		3B
TS17: endocardial cushion	Present		3B
TS17: outflow tract	Present	Regionally restricted	3B	Expression extended from the atrioventricular canal into the aortic sac.

Structure	Level	Pattern	Image	Note
TS20: endocardial cushion	Present		3C
TS20: outflow tract	Present	Regionally restricted	3C	Expression was detected in the aortic sac.

Structure	Level	Pattern	Image	Note
TS22: endocardial cushion	Present		3D
TS22: outflow tract	Present	Regionally restricted	3D	Expression was detected in the aortic sac.

Structure	Level	Pattern	Image	Note
TS23: endocardial cushion	Present		3E
TS23: heart	Present	Regionally restricted	3E	Expression was detected in the aortic sac and the endocardial cushions.

Structure	Level	Pattern	Image	Note
TS25: endocardial cushion	Present		3F
TS25: heart	Present	Regionally restricted	3F	Expression was detected in the aortic sac and the endocardial cushions.

Structure	Level	Pattern	Image	Note
TS20: trachea	Weak	Regionally restricted	4A	Expression was confined to the lining of the trachea and larger airways.

Structure	Level	Pattern	Image	Note
TS22: trachea	Weak	Regionally restricted	4D	Expression was confined to the lining of the trachea and larger airways.

Structure	Level	Pattern	Image	Note
TS23: trachea	Weak	Regionally restricted	4G	Expression was confined to the lining of the trachea and larger airways.

Structure	Level	Pattern	Image	Note
TS24: trachea	Weak	Regionally restricted	4J	Expression was confined to the lining of the trachea and larger airways.

Structure	Level	Pattern	Image	Note
TS25: trachea	Trace	Regionally restricted	4M	Expression was confined to the lining of the trachea and larger airways.

Structure	Level	Pattern	Image	Note
TS22: metanephros	Present	Regionally restricted	5A	Expression was largely confined to the maturing epithelial portions of the kidney.

Structure	Level	Pattern	Image	Note
TS23: renal pelvis	Present		5D
TS23: collecting duct	Present		5D

Structure	Level	Pattern	Image	Note
TS24: metanephros	Present	Regionally restricted	5G	Expression in the collecting ducts had virtually disappeared.

Structure	Level	Pattern	Image	Note
TS25: metanephros	Present		5J