Shh RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:83956 Bladt F, et al., The murine Nck SH2/SH3 adaptors are important for the development of mesoderm-derived embryonic structures and for regulating the cellular actin network. Mol Cell Biol. 2003 Jul;23(13):4586-97
Assay type:	RNA in situ
MGI Accession ID:	MGI:3663600
Gene symbol:	Shh
Gene name:	sonic hedgehog

Probe:	Shh probe54
Probe preparation:	Antisense RNA
Assay notes:	Fixative was 1.6% formaldehyde-0.2% glutaraldehyde.

Results

Specimen 3Aa: embryonic day 8.5; Nck1^tm1Paw/Nck1⁺, Nck2^tm1Paw/Nck2⁺ (more

)

Structure	Level	Image
TS13: neural tube floor plate	Present	3Aa
TS13: notochord	Present	3Aa
TS13: foregut diverticulum endoderm	Present	3Aa
TS13: hindgut diverticulum endoderm	Present	3Aa

Specimen 3Ac: embryonic day 8.5; Nck1^tm1Paw/Nck1^tm1Paw, Nck2^tm1Paw/Nck2^tm1Paw (more

)

Structure	Level	Pattern	Image	Note
TS13: notochord	Present		3Ac	Expression is in the notochord floorplate.

Specimen 3Ba: embryonic day 8.5; Nck1^tm1Paw/Nck1⁺, Nck2^tm1Paw/Nck2⁺ (more

)

Structure	Level	Pattern	Image	Note
TS13: neural tube floor plate	Present		3Ba
TS13: notochord	Present		3Ba

Specimen 3Bb: embryonic day 8.5; Nck1^tm1Paw/Nck1⁺, Nck2^tm1Paw/Nck2⁺ (more

)

Structure	Level	Image
TS13: gut endoderm	Present	3Bb
TS13: neural tube floor plate	Present	3Bb
TS13: notochord	Present	3Bb

Specimen 3Bc: embryonic day 8.5; Nck1^tm1Paw/Nck1⁺, Nck2^tm1Paw/Nck2⁺ (more

)

Structure	Level	Pattern	Image	Note
TS13: neural tube floor plate	Present		3Bc
TS13: notochord	Present		3Bc

Specimen 3Bd: embryonic day 8.5; Nck1^tm1Paw/Nck1^tm1Paw, Nck2^tm1Paw/Nck2^tm1Paw (more

)

Structure	Level	Pattern	Image	Note
TS13: neural tube floor plate	Present	Regionally restricted	3Bd	Abnormal fragmentation and bifurcation of staining pattern.
TS13: notochord	Present	Regionally restricted	3Bd	Abnormal fragmentation and bifurcation of staining pattern.

Specimen 3Be: embryonic day 8.5; Nck1^tm1Paw/Nck1^tm1Paw, Nck2^tm1Paw/Nck2^tm1Paw (more

)

Structure	Level	Pattern	Image	Note
TS13: neural tube floor plate	Present	Regionally restricted	3Be	Abnormal fragmentation and bifurcation of staining pattern.
TS13: notochord	Present	Regionally restricted	3Be	Abnormal fragmentation and bifurcation of staining pattern.

Specimen 3Bf: embryonic day 8.5; Nck1^tm1Paw/Nck1^tm1Paw, Nck2^tm1Paw/Nck2^tm1Paw (more

)

Structure	Level	Pattern	Image	Note
TS13: neural tube floor plate	Present	Regionally restricted	3Bf	Abnormal fragmentation and bifurcation of staining pattern.
TS13: notochord	Present	Regionally restricted	3Bf	Abnormal fragmentation and bifurcation of staining pattern.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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