Tg(CAG-DsRed*MST)1Nagy Transgene Detail MGI Mouse (MGI:3663358)

Tg(CAG-DsRed*MST)1Nagy
Transgene Detail

Summary

Symbol:	Tg(CAG-DsRed*MST)1Nagy
Name:	transgene insertion 1, Andras Nagy
MGI ID:	MGI:3663358
Synonyms:	Actb-DsRed.T3, CAG-DsRed, DsRed.T3, Tg(ACTB-DsRed.MST)1Nagy, Tg(ACTB-DsRedMST)1Nagy, Tg(ACTB-DsRedMTS)1Nagy
Transgene:	Tg(CAG-DsRed*MST)1Nagy Location: unknown
Alliance:	Tg(CAG-DsRed*MST)1Nagy page

Transgene
origin

Germline Transmission:	Earliest citation of germline transmission: J:109837
Parent Cell Line:	G4 (ES Cell)
Strain of Origin:	(129S6/SvEvTac x C57BL/6NCrl)F1

Transgene
description

Transgene Type:		Transgenic (Reporter)
Mutation:		Insertion
		Mutation details: This strain is a derivative of Tg(ACTB-Bgeo,-DsRedMST). A transgenic construct containing a Red Fluorescent Protein gene (DsRed.MST) under the control of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer upstream of a loxP site flanked beta-geo gene and polyadenylation sites, was introduced into 129S6B6F1 derived G4 embryonic stem (ES) cells. Then the expression of lacZ was replaced with red fluorescent protein following in vitro Cre recombination. All tissues of homozygotes fluoresce red. Mice hemizygous for the transgene express red fluorescent protein less intensely than homozygotes. Expression is observed throughout all embryonic and adult stages and very high expression is found in pancreas, skeletal muscle, heart and seminal vesicle. (J:109837*)

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 4 strains available Cell Lines: 0 lines available

Notes

Erratum publ Genesis 42:218 (2005) correction to original (2004) publication: The red fluorescent protein used is DsRed.MST, not DsRed.T3 as J:102837. DsRed.MST differs from DsRed.T3 in two amino acids. It has Alanine at positions 145 and 217.

References

Original:	J:109837 Vintersten K, et al., Mouse in red: red fluorescent protein expression in mouse ES cells, embryos, and adult animals. Genesis. 2004 Dec;40(4):241-6
All:	116 reference(s)

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