Summary | Transgene origin | Transgene description | Find Mice (IMSR) | References

Symbol:	Tg(CAG-mRFP1)1F1Hadj
Name:	transgene insertion 1F1, Anna-Katerina Hadjantonakis
MGI ID:	MGI:3663356
Synonyms:	CAG::mRFP1, Tg(ACTB-mRFP1)1F1Hadj
Transgene:	Tg(CAG-mRFP1)1F1Hadj  Location: unknown
Alliance:	Tg(CAG-mRFP1)1F1Hadj page

Germline Transmission:	Earliest citation of germline transmission: J:104237
Parent Cell Line:	R1 (ES Cell)
Strain of Origin:	(129X1/SvJ x 129S1/Sv)F1-Kitl+

Transgene Type:    Transgenic (Reporter) Mutation:    Insertion   Mutation details: The first true monomeric red fluorescent protein (mRFP1) is the result of the stepwise directed evolution of DsRed; introducing 33 amino acid substitutions selecting for improved performance and developmental neutrality. The mRFP1 coding sequence was placed under control of the chicken beta actin promoter and cytomegalovirus (CMV) immediate early enhancer combination, designed to drive high-level constitutive gene expression in ES cells, embryos and adult mice. The construct (CAG-mRFP1) was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Mice hemizygous for the transgene exhibit robust and widespread expression of monomeric red fluorescent protein-1 (mRFP1) in embryonic stem cells, embryos, and adults. (J:104237)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 2 strains available      Cell Lines: 0 lines available

Original:	J:104237 Long JZ, et al., Genetic and spectrally distinct in vivo imaging: embryonic stem cells and mice with widespread expression of a monomeric red fluorescent protein. BMC Biotechnol. 2005 Jul 4;5:20
All:	29 reference(s)