Gene Expression Data
Assay Details
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Assay
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Reference:
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J:106765
van Wijk E, et al., The DFNB31 gene product whirlin connects to the Usher protein network in the cochlea and retina by direct association with USH2A and VLGR1. Hum Mol Genet. 2006 Mar 1;15(5):751-65
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3653115
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Gene symbol:
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Whrn
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Gene name:
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whirlin
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Probe:
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Whrn cDNA
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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The probe used in this assay recognizes all reported splice varients.
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Results
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Specimen
3A:
embryonic day 10.5
(more  )
Note: This section was post-fixed in 4% paraformaldehyde.
Specimen
3A:
(close  )
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Genetic Background:
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involves: C57BL/6J
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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This section was post-fixed in 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: brain |
Present |
Regionally restricted |
3A
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Expression is present in the neural epithelium, with the strongest staining in the ventral neural epithelium.
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| TS17: neural tube lateral wall |
Present |
Regionally restricted |
3A
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Expression is present in the basal plate of the spinal cord.
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| TS17: lung |
Present |
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3A
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Specimen
3B:
embryonic day 12.5
(more )
Note: This section was post-fixed in 4% paraformaldehyde.
Specimen
3B:
(close )
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Genetic Background:
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involves: C57BL/6J
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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This section was post-fixed in 4% paraformaldehyde.
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Specimen
3C:
embryonic day 14.5
(more )
Note: This section was post-fixed in 4% paraformaldehyde.
Specimen
3C:
(close )
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Genetic Background:
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involves: C57BL/6J
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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This section was post-fixed in 4% paraformaldehyde.
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Specimen
3D:
embryonic day 12.5
(more )
Note: This section was post-fixed in 4% paraformaldehyde.
Specimen
3D:
(close )
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Genetic Background:
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involves: C57BL/6J
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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This section was post-fixed in 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: neural retinal epithelium |
Present |
Regionally restricted |
3D
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Expression is present in the inner neuroblastic layer of the retina.
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Specimen
3E:
embryonic day 14.5
(more )
Note: This section was post-fixed in 4% paraformaldehyde.
Specimen
3E:
(close )
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Genetic Background:
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involves: C57BL/6J
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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This section was post-fixed in 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: neural retinal epithelium |
Strong |
Regionally restricted |
3E
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Expression is present in the inner neuroblastic layer of the retina.
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Specimen
3F:
embryonic day 16.5
(more )
Note: This section was post-fixed in 4% paraformaldehyde.
Specimen
3F:
(close )
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Genetic Background:
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involves: C57BL/6J
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Age:
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embryonic day 16.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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This section was post-fixed in 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: neural retina |
Present |
Regionally restricted |
3F
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Expression is present in the inner neuroblastic layer of the retina.
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Specimen
3G:
embryonic day 18.5
(more )
Note: This section was post-fixed in 4% paraformaldehyde.
Specimen
3G:
(close )
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Genetic Background:
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involves: C57BL/6J
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Age:
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embryonic day 18.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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This section was post-fixed in 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neural retina |
Present |
Regionally restricted |
3G
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Expression is limited to a subset of the ganglion cells. Expression is not detected in the innermost region of these layers.
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Specimen
3H:
postnatal day 7
(more )
Specimen
3H:
(close )
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Genetic Background:
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involves: C57BL/6J
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Specimen
3I:
postnatal day 90
(more )
Specimen
3I:
(close )
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Genetic Background:
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involves: C57BL/6J
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Age:
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postnatal day 90
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Sex:
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Not Specified
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Type:
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section
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Specimen
3J:
embryonic day 14.5
(more )
Note: This section was post-fixed in 4% paraformaldehyde.
Specimen
3J:
(close )
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Genetic Background:
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involves: C57BL/6J
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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This section was post-fixed in 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: utricle |
Present |
Regionally restricted |
3J
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Expression is present in the crista of the semicircular canal and in the maculae of the utricle.
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| TS22: saccule |
Present |
Regionally restricted |
3J
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Expression is present in the developing maculae.
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| TS22: vestibulo-cochlear ganglion |
Present |
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3J
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Specimen
3K:
embryonic day 16.5
(more )
Note: This section was post-fixed in 4% paraformaldehyde.
Specimen
3K:
(close )
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Genetic Background:
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involves: C57BL/6J
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Age:
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embryonic day 16.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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This section was post-fixed in 4% paraformaldehyde.
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Specimen
3L:
embryonic day 18.5
(more )
Note: This section was post-fixed in 4% paraformaldehyde.
Specimen
3L:
(close )
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Genetic Background:
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involves: C57BL/6J
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Age:
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embryonic day 18.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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This section was post-fixed in 4% paraformaldehyde.
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Specimen
Not shown:
embryonic day 16.5
(more )
Note: This section was post-fixed in 4% paraformaldehyde.
Specimen
Not shown:
(close )
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Genetic Background:
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involves: C57BL/6J
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Age:
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embryonic day 16.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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This section was post-fixed in 4% paraformaldehyde.
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Specimen
Not shown:
embryonic day 18.5
(more )
Note: This section was post-fixed in 4% paraformaldehyde.
Specimen
Not shown:
(close )
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Genetic Background:
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involves: C57BL/6J
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Age:
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embryonic day 18.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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This section was post-fixed in 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cochlear duct epithelium |
Trace |
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There is very weak expression in the developing neurosensory epithelium of the cochlea.
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