Gene Expression Data
Assay Details
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Assay
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Reference:
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J:107704
Xiang M, Requirement for Brn-3b in early differentiation of postmitotic retinal ganglion cell precursors. Dev Biol. 1998 May 15;197(2):155-69
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3641278
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Gene symbol:
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Ebf1
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Gene name:
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early B cell factor 1
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Antibody:
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Anti-Olf-1
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin
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Results
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Specimen
3M:
(close  )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J
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Mutant Allele(s):
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Pou4f2tm1Nat/Pou4f2+
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3N:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J
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Mutant Allele(s):
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Pou4f2tm1Nat/Pou4f2tm1Nat
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
6A:
embryonic day 12.5; Pou4f2tm1Nat/Pou4f2 +
(more  )
Specimen
6A:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J
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Mutant Allele(s):
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Pou4f2tm1Nat/Pou4f2+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: retina |
Present |
Regionally restricted |
6A anti-Olf-1
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Expression was detected in the ganglion cell layer of the central region of the retina.
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Specimen
6B:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J
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Mutant Allele(s):
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Pou4f2tm1Nat/Pou4f2tm1Nat
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: retina |
Present |
Regionally restricted |
6B anti-Olf-1
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There were 4-6 fold fewer labeled cells in the mutant retina compared to control.
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Specimen
6C:
embryonic day 13.5; Pou4f2tm1Nat/Pou4f2 +
(more )
Specimen
6C:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J
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Mutant Allele(s):
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Pou4f2tm1Nat/Pou4f2+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: retina |
Present |
Regionally restricted |
6C anti-Olf-1
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Expression was detected in ganglion cells.
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Specimen
6D:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J
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Mutant Allele(s):
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Pou4f2tm1Nat/Pou4f2tm1Nat
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: retina |
Present |
Regionally restricted |
6D anti-Olf-1
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There were 4-6 fold fewer labeled cells in the mutant retina compared to control.
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Specimen
6E:
embryonic day 15.5; Pou4f2tm1Nat/Pou4f2 +
(more )
Specimen
6E:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J
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Mutant Allele(s):
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Pou4f2tm1Nat/Pou4f2+
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: retina |
Present |
Regionally restricted |
6E anti-Olf-1
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Expression was detected in ganglion cells.
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Specimen
6F:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J
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Mutant Allele(s):
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Pou4f2tm1Nat/Pou4f2tm1Nat
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: retina |
Present |
Regionally restricted |
6F anti-Olf-1
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There were 4-6 fold fewer labeled cells in the mutant retina compared to control.
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Specimen
6G:
embryonic day 18.5; Pou4f2tm1Nat/Pou4f2 +
(more )
Specimen
6G:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J
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Mutant Allele(s):
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Pou4f2tm1Nat/Pou4f2+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: retina |
Present |
Regionally restricted |
6G anti-Olf-1
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Expression was detected in ganglion cells.
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Specimen
6H:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J
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Mutant Allele(s):
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Pou4f2tm1Nat/Pou4f2tm1Nat
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: retina |
Present |
Regionally restricted |
6H anti-Olf-1
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There were 4-6 fold fewer labeled cells in the mutant retina compared to control.
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Analysis Tools