Gene Expression Data
Assay Details
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Assay
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Reference:
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J:88430
Kim YS, et al., GLIS3, a novel member of the GLIS subfamily of Kruppel-like zinc finger proteins with repressor and activation functions. Nucleic Acids Res. 2003 Oct 1;31(19):5513-25
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3640372
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Gene symbol:
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Glis3
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Gene name:
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GLIS family zinc finger 3
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Probe:
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Glis3 cDNA1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Assay notes:
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It is possible another probe (MGI:3640362) was used in this assay.
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Results
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Specimen
Not shown:
embryonic day 6.5
(more  )
Specimen
Not shown:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 6.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
4A:
embryonic day 8.0
(more )
Specimen
4A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: node |
Present |
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4A
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Specimen
4B:
embryonic day 8.25
(more )
Specimen
4B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.25
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: primitive streak |
Present |
Regionally restricted |
4B
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Expression is present in the node.
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| TS12: future hindbrain |
Present |
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4B
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Expression is present in the neural plate of the presumptive hindbrain.
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Specimen
4C:
embryonic day 8.5
(more )
Note: This is a lateral view.
Specimen
4C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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This is a lateral view.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: neural ectoderm |
Present |
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4C
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Expression is restricted to the neural epithelium; it is prominent in some regions.
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| TS13: future hindbrain |
Present |
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4C
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Expression is prominent in the hindbrain region and the cephalic flexure of the presumptive mid/hindbrain.
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| TS13: future midbrain |
Present |
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4C
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Expression is prominent in the hindbrain region and the cephalic flexure of the presumptive mid/hindbrain.
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| TS13: future prosencephalon |
Present |
Regionally restricted |
4C
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Expression is present in the anterior forebrain.
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Specimen
4D:
embryonic day 8.75
(more )
Note: This is a lateral view.
Specimen
4D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.75
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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This is a lateral view.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: future hindbrain |
Present |
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4D
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Diffuse expression is present in the hindbrain region and in the cephalic flexure of the presumptive mid/hindbrain.
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| TS14: future midbrain |
Present |
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4D
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Diffuse expression is present in the hindbrain region and in the cephalic flexure of the presumptive mid/hindbrain.
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| TS14: prosencephalon |
Present |
Regionally restricted |
4D
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There is diffuse expression in the anterior forebrain.
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| TS14: neural tube |
Present |
Regionally restricted |
4D
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Expression is present in the dorsal neural tube.
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Specimen
4D inset:
embryonic day 8.75
(more )
Specimen
4D inset:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.75
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: otic placode |
Present |
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4D inset
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Expression is present in the anterior/dorsal region of the otic vesicles.
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Specimen
4E:
embryonic day 9.5
(more )
Note: This is a lateral view.
Specimen
4E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Theiler stage of this specimen assigned by curators from EMAGE. Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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This is a lateral view.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: forelimb bud mesenchyme |
Present |
Regionally restricted |
4E
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Expression is present in the proximal central limb bud mesenchyme; similar expression was seen in the hindlimb later in development.
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| TS16: midbrain-hindbrain junction |
Present |
Regionally restricted |
4E
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Expression is present in the isthmus.
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| TS16: neural tube |
Present |
Regionally restricted |
4E
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Expression is present in the dorsal neural tube.
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| TS16: future forebrain |
Present |
Regionally restricted |
4E
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Expression is present in the basal plate of the forebrain.
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Specimen
4F:
embryonic day 10.5
(more )
Note: This is a lateral view.
Specimen
4F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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This is a lateral view.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube roof plate |
Present |
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4F
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| TS17: head |
Present |
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4F
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Expression is evident around the nasal placodes.
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| TS17: limb mesenchyme |
Present |
Regionally restricted |
4F
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Expression is restricted to the anterior proximal mesenchyme; apparent differences between expression in the fore and hind limbs are because the forelimb is developmentally more advanced.
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Specimen
4G:
embryonic day 11.5
(more )
Note: This is a transverse section.
Specimen
4G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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This is a transverse section.
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Specimen
4H:
embryonic day 11.5
(more )
Note: This is a lateral view.
Specimen
4H:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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This is a lateral view.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: head mesenchyme |
Present |
Regionally restricted |
4H
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Expression is present in the mesenchyme of the face.
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| TS19: footplate |
Strong |
Regionally restricted |
4H
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There is strong expression in the interdigital region.
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| TS19: handplate |
Strong |
Regionally restricted |
4H
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There is strong expression in the interdigital region.
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Specimen
4I:
embryonic day 12.5
(more )
Note: This is a frontal view.
Specimen
4I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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This is a frontal view.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: head mesenchyme |
Present |
Regionally restricted |
4I
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Expression is present in the mesenchyme of the face.
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Specimen
4J:
embryonic day 12.5
(more )
Note: This is a frontal view.
Specimen
4J:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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This is a frontal view.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: telencephalon |
Strong |
Regionally restricted |
4J
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Expression is present in the medial walls of the two telencephalic vesicles.
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| TS20: forebrain |
Present |
Regionally restricted |
4J
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Expression is present in the eminentia thalami, which connects the dorsal telencephalon and diencephalon.
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Specimen
5A:
embryonic day 9.5
(more )
Note: This is a frontal section.
Specimen
5A:
(close )
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Genetic Background:
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Not Specified
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Age:
|
embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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This is a frontal section.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: optic vesicle |
Present |
Regionally restricted |
5A
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Expression is present in the ventral optic vesicle.
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| TS15: surface ectoderm |
Present |
Regionally restricted |
5A
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Expression is present in the surface ectoderm of the head near the optic vesicles.
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Specimen
5B:
embryonic day 10.5
(more )
Note: This is a lateral view.
Specimen
5B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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|
Note:
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This is a lateral view.
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Specimen
5C:
embryonic day 10.5
(more )
Note: This is a view from the bottom of the head.
Specimen
5C:
(close )
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Genetic Background:
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Not Specified
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Age:
|
embryonic day 10.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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|
Note:
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This is a view from the bottom of the head.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: optic cup |
Present |
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5C
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| TS17: optic stalk |
Present |
Regionally restricted |
5C
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Expression is present in the nasal portion of the optic stalk.
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Specimen
Not shown:
embryonic day 11.5
(more )
Specimen
Not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
|
embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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4% Paraformaldehyde
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Specimen
Not shown:
embryonic day 12.5
(more )
Specimen
Not shown:
(close )
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Genetic Background:
|
Not Specified
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Age:
|
embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: eye |
Absent |
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Expression is not evident in any optic structures.
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Specimen
5D:
embryonic day 14.5
(more )
Specimen
5D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
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Type:
|
whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5E:
embryonic day 14.5
(more )
Specimen
5E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Male
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5F:
embryonic day 14.5
(more )
Specimen
5F:
(close )
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Genetic Background:
|
Not Specified
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Age:
|
embryonic day 14.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: collecting duct |
Present |
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5F
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Expression is present in the branches of the ureteric bud.
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Specimen
5F inset:
embryonic day 14.5
(more )
Specimen
5F inset:
(close )
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Genetic Background:
|
Not Specified
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Age:
|
embryonic day 14.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: collecting duct |
Present |
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5F inset
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Expression is present in the branches of the ureteric bud.
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Specimen
5G:
embryonic day 12.5
(more )
Note: This is a view from the anterior perspective.
Specimen
5G:
(close )
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Genetic Background:
|
Not Specified
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Age:
|
embryonic day 12.5
|
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
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Sex:
|
Not Specified
|
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Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
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This is a view from the anterior perspective.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: genital tubercle |
Present |
Regionally restricted |
5G
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Expression in present in lateral mesenchymal stripes in the genital tubercle.
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Specimen
5H:
embryonic day 14.5
(more )
Note: This is a view from the anterior perspective.
Specimen
5H:
(close )
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Genetic Background:
|
Not Specified
|
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Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
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Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
This is a view from the anterior perspective.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: reproductive system |
Present |
Regionally restricted |
5H
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Expression is present in the preputial swellings, which are outgrowths lateral to the genital tubercle that will form the prepuce of the external genitalia.
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Specimen
5I:
embryonic day 12.5
(more )
Note: This specimen's anterior aspect is facing up.
Specimen
5I:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 12.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
This specimen's anterior aspect is facing up.
|
Specimen
5J:
embryonic day 14.5
(more )
Note: This specimen's anterior aspect is facing up.
Specimen
5J:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
This specimen's anterior aspect is facing up.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: forelimb digit |
Present |
Regionally restricted |
5J
|
Expression is detected along the digits, in the interphalangeal joints of each digit, and in muscles of the hand plate and proximal digit.
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| TS22: hand |
Present |
Regionally restricted |
5J
|
Expression is detected along the digits, in the interphalangeal joints of each digit, and in muscles of the hand plate and proximal digit.
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| TS22: hand mesenchyme |
Present |
Regionally restricted |
5J
|
Expression is detected along the digits, in the interphalangeal joints of each digit, and in muscles of the hand plate and proximal digit.
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Analysis Tools