Gene Expression Data
Assay Details
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Assay
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Reference:
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J:109156
Liu H, et al., Identification and developmental expression analysis of a novel homeobox gene closely linked to the mouse Twirler mutation. Gene Expr Patterns. 2006 Aug;6(6):632-6
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3639503
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Gene symbol:
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Mkx
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Gene name:
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mohawk homeobox
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Probe:
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Irxl1 cDNA1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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The authors did not specify which probe was used, they could have used Irxl1 cDNA2 (MGI:3639501). They state that both probes detected identical expression patterns during mouse embryogenesis in both whole mount and section in situ hybridizationassays.
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Results
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Specimen
2A:
embryonic day 10.0
(more  )
Specimen
2A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: trunk dermomyotome |
Present |
Regionally restricted |
2A
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Expression is in dermomyotomes at the forelimb level.
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Specimen
2B:
embryonic day 10.0
(more )
Specimen
2B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: trunk dermomyotome |
Present |
Regionally restricted |
2B
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Expression is in dermomyotomes at the forelimb level.
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Specimen
2C:
embryonic day 11.5
(more )
Specimen
2C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: trunk dermomyotome |
Present |
Regionally restricted |
2C
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Expresssion is in the dorsal and ventral domains in the dermomyotomes between the fore- and hind-limb buds.
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| TS19: limb |
Strong |
Regionally restricted |
2C
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Expression is in myogenic precursor cells migrating into the limb buds.
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| TS19: trunk mesenchyme |
Strong |
Regionally restricted |
2C
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Expression is in myogenic precursor cells migrating into the limb buds.
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| TS19: head mesenchyme |
Strong |
Regionally restricted |
2C
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Expression is in a region ventral to the telencephalic vesicles.
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Specimen
2D:
embryonic day 11.75
(more )
Specimen
2D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.75
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: limb |
Strong |
Regionally restricted |
2D
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Expression is in myogenic precursor cells migrating into the limb buds.
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| TS20: trunk mesenchyme |
Strong |
Regionally restricted |
2D
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Expression is in myogenic precursor cells migrating into the limb buds.
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| TS20: head mesenchyme |
Strong |
Regionally restricted |
2D
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Expression is in a region ventral to the telencephalic vesicles.
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Specimen
2E:
embryonic day 11.75
(more )
Specimen
2E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.75
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: head mesenchyme |
Strong |
Regionally restricted |
2E
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Expression is in a region ventral to the telencephalic vesicles.
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Specimen
2F:
embryonic day 12.5
(more )
Specimen
2F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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