Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Pik3catm1Bvan
Name:	phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha; targeted mutation 1, Bart Vanhaesebroeck
MGI ID:	MGI:3628805
Synonyms:	p110alphaD933A, p110alpha KI
Gene:	Pik3ca  Location: Chr3:32451203-32520256 bp, + strand  Genetic Position: Chr3, 15.7 cM, cytoband B
Alliance:	Pik3catm1Bvan page

Germline Transmission:	Earliest citation of germline transmission: J:109024
Parent Cell Line:	E14TG2a (ES Cell)
Strain of Origin:	129P2/OlaHsd

Allele Type:    Targeted Mutations:    Insertion, Nucleotide substitutions   Mutation details: This allele is derived from Pik3ca and was produced as follows: A point mutation was engineered into the locus, resulting in the DFG to AFG conversion in the conserved DFG motif at the C-terminal kinase domain. A C-terminal Myc-epitope tag was introduced immediately preceding the translational stop codon as well as a floxed IRES-lacZ-geo cassette in the 3' UTR. The floxed sequence was subsequently removed by crossing with mice carrying the Tg(CMV-cre)1Cgn transgene. The p110alpha, p110beta, and p110gamma subunit expression was not altered in homozygous embryos and in liver, muscle and fat from hetrozygous mice. The heterozygous mutant demonstrated 50% loss of function in overall class IA PI(3)K activity in liver, muscle and fat. (J:109024)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

5 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Pik3ca Mutation:	62 strains or lines available

Original:	J:109024 Foukas LC, et al., Critical role for the p110alpha phosphoinositide-3-OH kinase in growth and metabolic regulation. Nature. 2006 May 18;441(7091):366-70
All:	14 reference(s)