Gene Expression Data
Assay Details
Assay
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Reference:
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J:48282
Kohmura N, et al., Diversity revealed by a novel family of cadherins expressed in neurons at a synaptic complex. Neuron. 1998 Jun;20(6):1137-51
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3628539
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Gene symbol:
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Pcdha4
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Gene name:
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protocadherin alpha 4
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Antibody:
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6-1B
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Detection system:
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Secondary antibody coupled to other - see notes
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Assay notes:
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Immunoelectron microscopy with ultrathin sections. Secondary antibody was a gold-coupled anti-mouse IgM. Fixative was 2.5% or 1% glutaraldehyde, 0.5% paraformaldehyde, and 0.1% picric acid in 0.1M phosphate buffer. Embedded in Epon 812.
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Results
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Specimen
4C,D:
postnatal week 3
(more )
Specimen
4C,D:
(close )
Genetic Background:
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CD-1
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Age:
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postnatal week 3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Resin/plastic
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Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebral cortex |
Present |
Regionally restricted |
4D
,
4C
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Expression is at the synaptic junctional complexes.
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Specimen
4E:
postnatal week 3
(more )
Specimen
4E:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal week 3
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Resin/plastic
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebral cortex |
Present |
Regionally restricted |
4E
|
Expression is at the synaptic junctional complexes.
|
Specimen
4F:
postnatal week 3
(more )
Specimen
4F:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal week 3
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Resin/plastic
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebral cortex |
Present |
Regionally restricted |
4F
|
Expression is at the synaptic junctional complexes.
|
Specimen
4G:
postnatal week 3
(more )
Specimen
4G:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal week 3
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Resin/plastic
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebral cortex |
Present |
Regionally restricted |
4G
|
Expression is at the synaptic junctional complexes.
|
|