Summary | Transgene origin | Transgene description | Find Mice (IMSR) | References

Symbol:	Tg(Zp3-EGFP)1Dean
Name:	transgene insertion 1, Jurrien Dean
MGI ID:	MGI:3624514
Synonyms:	EGFP-MoZP3, EGFP-ZP3, EGFP-ZP3 (normal furin site), normal ZP3-EGFP
Transgene:	Tg(Zp3-EGFP)1Dean  Location: unknown
Alliance:	Tg(Zp3-EGFP)1Dean page

Strain of Origin:

FVB/N

Transgene Type:    Transgenic (Reporter) Mutation:    Insertion   Mutation details: The ~8.1 kb transgene comprises the mouse Zp3 cDNA into which the the enhanced green fluorescent protein (EGFP) cDNA has been inserted downstream of the sequence encoding the 40 amino acid N-terminal signal peptide. Expression of the fusion gene is driven by ~6 kb of the oocyte-specific mouse Zp3 promoter. Ovulated ova and growing oocytes from transgenic mice exhibit EGFP fluorescence throughout the matrix of the zona pellucida; surrounding somatic cells do not fluoresce. Mature oocytes exhibit the strongest signal. Smaller, growing oocytes contain large, circular structures whose boundaries fluoresce and costain with BODIPY-Texas red ceramide. (J:78932, J:86870)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 2 strains available      Cell Lines: 0 lines available

Original:	J:78932 Zhao M, et al., Conserved furin cleavage site not essential for secretion and integration of ZP3 into the extracellular egg coat of transgenic mice. Mol Cell Biol. 2002 May;22(9):3111-20
All:	3 reference(s)