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Gene Expression Data
Assay Details
Assay
Reference: J:102872 McGlinn E, et al., Pax9 and Jagged1 act downstream of Gli3 in vertebrate limb development. Mech Dev. 2005 Nov;122(11):1218-33
Assay type: Northern blot
MGI Accession ID: MGI:3618220
Gene symbol: Prrx1
Gene name: paired related homeobox 1
Probe: Prrx1 cDNA1
Probe preparation: Double stranded DNA
Visualized with: Phosphorimaging
Assay notes: Bands were quantified using ImageQuant and standardized for loading using GAPDH as an internal control. Within each sample, expression levels are presented as ratios relative to wild-type, arbitrarily defined as 1.
Results
Image: 3B ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
1.0 E11.5 (a) TS19: hindlimb bud Present 8 µg; total RNA Not Specified Not Specified
0.96 E11.5 (a) TS19: hindlimb bud Present 8 µg; total RNA involves: C3H/HeJ * C57BL/6 Gli3Xt-J/Gli3+Not Specified
0.64 E11.5 (a) TS19: hindlimb bud Weak 8 µg; total RNA involves: C3H/HeJ * C57BL/6 Gli3Xt-J/Gli3Xt-JNot Specified
1.0 E11.5 (a) TS19: forelimb bud Present 8 µg; total RNA Not Specified Not Specified
0.89 E11.5 (a) TS19: forelimb bud Present 8 µg; total RNA involves: C3H/HeJ * C57BL/6 Gli3Xt-J/Gli3+Not Specified
0.52 E11.5 (a) TS19: forelimb bud Weak 8 µg; total RNA involves: C3H/HeJ * C57BL/6 Gli3Xt-J/Gli3Xt-JNot Specified
1.0 E12.5 (a) TS20: hindlimb Present 8 µg; total RNA Not Specified Not Specified
0.91 E12.5 (a) TS20: hindlimb Present 8 µg; total RNA involves: C3H/HeJ * C57BL/6 Gli3Xt-J/Gli3+Not Specified
0.57 E12.5 (a) TS20: hindlimb Weak 8 µg; total RNA involves: C3H/HeJ * C57BL/6 Gli3Xt-J/Gli3Xt-JNot Specified
1.0 E12.5 (a) TS20: forelimb Present 8 µg; total RNA Not Specified Not Specified
0.90 E12.5 (a) TS20: forelimb Present 8 µg; total RNA involves: C3H/HeJ * C57BL/6 Gli3Xt-J/Gli3+Not Specified
0.63 E12.5 (a) TS20: forelimb Weak 8 µg; total RNA involves: C3H/HeJ * C57BL/6 Gli3Xt-J/Gli3Xt-JNot Specified
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory