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Gene Expression Data
Assay Details
Assay
Reference: J:102872 McGlinn E, et al., Pax9 and Jagged1 act downstream of Gli3 in vertebrate limb development. Mech Dev. 2005 Nov;122(11):1218-33
Assay type: Northern blot
MGI Accession ID: MGI:3618208
Gene symbol: Acan
Gene name: aggrecan
Probe: Agc1 cDNA3
Probe preparation: Double stranded DNA
Visualized with: Phosphorimaging
Assay notes: Bands were quantified using ImageQuant and standardized for loading using GAPDH as an internal control. Within each sample, expression levels are presented as ratios relative to wild-type, arbitrarily defined as 1.
Results
Image: 3D ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
Lane 1 E11.5 (a) TS19: hindlimb bud Absent 8 µg; total RNA Not Specified Not Specified
Lane 2 E11.5 (a) TS19: hindlimb bud Absent 8 µg; total RNA involves: C3H/HeJ * C57BL/6 Gli3Xt-J/Gli3+Not Specified
Lane 3 E11.5 (a) TS19: hindlimb bud Absent 8 µg; total RNA involves: C3H/HeJ * C57BL/6 Gli3Xt-J/Gli3Xt-JNot Specified
Lane 4 E11.5 (a) TS19: forelimb bud Absent 8 µg; total RNA Not Specified Not Specified
Lane 5 E11.5 (a) TS19: forelimb bud Absent 8 µg; total RNA involves: C3H/HeJ * C57BL/6 Gli3Xt-J/Gli3+Not Specified
Lane 6 E11.5 (a) TS19: forelimb bud Absent 8 µg; total RNA involves: C3H/HeJ * C57BL/6 Gli3Xt-J/Gli3Xt-JNot Specified
1.0 E12.5 (a) TS20: hindlimb Present 8 µg; total RNA Not Specified Not Specified
1.06 E12.5 (a) TS20: hindlimb Present 8 µg; total RNA involves: C3H/HeJ * C57BL/6 Gli3Xt-J/Gli3+Not Specified
0.71 E12.5 (a) TS20: hindlimb Present 8 µg; total RNA involves: C3H/HeJ * C57BL/6 Gli3Xt-J/Gli3Xt-JNot Specified
1.0 E12.5 (a) TS20: forelimb Present 8 µg; total RNA Not Specified Not Specified
0.92 E12.5 (a) TS20: forelimb Present 8 µg; total RNA involves: C3H/HeJ * C57BL/6 Gli3Xt-J/Gli3+Not Specified
0.63 E12.5 (a) TS20: forelimb Present 8 µg; total RNA involves: C3H/HeJ * C57BL/6 Gli3Xt-J/Gli3Xt-JNot Specified
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
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