Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:103446
McKee AE, et al., A genome-wide in situ hybridization map of RNA-binding proteins reveals anatomically restricted expression in the developing mouse brain. BMC Dev Biol. 2005 Jul 20;5:14
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:3618163
|
|
Gene symbol:
|
U2af2
|
|
Gene name:
|
U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 2
|
|
Probe:
|
U2af2 cDNA1
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
Assay notes:
|
The results of this assay were listed in Additional File 3, U2af2.
|
|
|
Results
|
Specimen
E13.5:
embryonic day 13.5
(more  )
Note: Authors indicate that there was either ubiquitous expression that is difficult to distinguish from background, or no expression. The authors observed uneven signals in different brain regions and annotated some structures as "Present".
Specimen
E13.5:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 13.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Authors indicate that there was either ubiquitous expression that is difficult to distinguish from background, or no expression. The authors observed uneven signals in different brain regions and annotated some structures as "Present".
|
Specimen
P0:
postnatal day 0
(more )
Note: Authors indicate that there was restricted expression in the nervous system with distinguishable expression in non-neural tissue.
Specimen
P0:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
postnatal day 0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Authors indicate that there was restricted expression in the nervous system with distinguishable expression in non-neural tissue.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: cerebral cortex |
Present |
|
R1871-21
,
R1871-22
|
|
| TS27: corpus striatum |
Present |
|
R1871-21
,
R1871-22
|
|
| TS27: hindbrain |
Present |
|
R1871-21
,
R1871-22
|
|
| TS27: hippocampus |
Present |
|
R1871-21
,
R1871-22
|
|
| TS27: hypothalamus |
Present |
|
R1871-21
,
R1871-22
|
|
| TS27: main olfactory bulb |
Present |
|
R1871-21
,
R1871-22
|
|
| TS27: midbrain |
Present |
|
R1871-21
,
R1871-22
|
|
| TS27: olfactory epithelium |
Present |
|
R1871-21
,
R1871-22
|
|
| TS27: retina |
Present |
|
R1871-21
,
R1871-22
|
|
| TS27: spinal cord |
Present |
|
R1871-21
,
R1871-22
|
|
| TS27: thalamus |
Present |
|
R1871-21
,
R1871-22
|
|
| TS27: trigeminal ganglion |
Present |
|
R1871-21
,
R1871-22
|
|
|
Analysis Tools