Tg(Nr2e1)5Ems
Transgene Detail
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| Symbol: |
Tg(Nr2e1)5Ems |
| Name: |
transgene insertion 5, Elizabeth M Simpson |
| MGI ID: |
MGI:3616884 |
| Synonyms: |
Tg(CITB-551D6)5Ems, Tg(Nr2e1bEMS4A)5Ems |
| Transgene: |
Tg(Nr2e1)5Ems Location: unknown Genetic Position: Chr16, Syntenic
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| Alliance: |
Tg(Nr2e1)5Ems page
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| Transgene Type: |
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Transgenic (Inserted expressed sequence) |
| Mutation: |
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Insertion
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Tg(Nr2e1)5Ems expresses
1 gene
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Mutation details: Southern blot analysis and interphase fluorescent in situ hybridization (FISH) both showed integration on Chr 16 of approximately 40 copies of the 194-kb BAC clone 551D6 from the CITB mouse library, which was made from 129S1/Sv-derived CJ7 embryonic stem cells. This clone contains a 187.2-kb insert that encompasses the entire Nr2e1 gene. Allele-specific RT-PCR of brain mRNA from C57BL/6J mice congenic for the transgene demonstrated expression of the 129S1/Sv-derived transgenic allele at backcross generation N10.
(J:105597)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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5 independent transgenic lines were established; the others are lines B/6Ems, C/7Ems, D/8Ems and E/9Ems.
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| Original: |
J:105597 Abrahams BS, et al., Metaphase FISHing of transgenic mice recommended: FISH and SKY define BAC-mediated balanced translocation. Genesis. 2003 Jul;36(3):134-41 |
| All: |
1 reference(s) |
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Analysis Tools
