TgTn(sb-lacZ,GFP)T4Jtak
Transgene Detail
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Symbol: |
TgTn(sb-lacZ,GFP)T4Jtak |
Name: |
transgenic transposon concatemer T4, Junji Takeda |
MGI ID: |
MGI:3613186 |
Transgene: |
TgTn(sb-lacZ,GFP)T4Jtak Location: unknown
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Alliance: |
TgTn(sb-lacZ,GFP)T4Jtak page
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Strain of Origin: |
(C57BL x DBA)F2
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Transgene Type: |
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Transgenic (Transposon concatemer) |
Mutation: |
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Insertion
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Mutation details: A concatemer of sleeping beauty transposable elements each consisting of a floxed PGK-neo cassette followed by inverted repeat direct repeat sequence specific for sleeping beauty transposase flanking human BCL2 splice acceptor sequence, IRES, lacZ, poly A signal, a chimeric CAG promoter containing sequence from the chicken ACTB promoter, GFP, and splice donor sequence. Expression of GFP is not seen in the concatemer (in 7 of 8 founders). Transposition inside a gene should result in ubiquitous expression of GFP by the ACTB promoter and expression of lacZ driven by the endogenous promoter. Transposition by sleeping beauty transposase does not move the PGK-neo cassette.
(J:104039)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Original: |
J:104039 Horie K, et al., Characterization of Sleeping Beauty transposition and its application to genetic screening in mice. Mol Cell Biol. 2003 Dec;23(24):9189-207 |
All: |
1 reference(s) |
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