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TgTn(sb-lacZ,GFP)T4Jtak
Transgene Detail
Summary
Symbol: TgTn(sb-lacZ,GFP)T4Jtak
Name: transgenic transposon concatemer T4, Junji Takeda
MGI ID: MGI:3613186
Transgene: TgTn(sb-lacZ,GFP)T4Jtak  Location: unknown  
Alliance: TgTn(sb-lacZ,GFP)T4Jtak page
Transgene
origin
Strain of Origin:  (C57BL x DBA)F2
Transgene
description
Transgene Type:    Transgenic (Transposon concatemer)
Mutation:    Insertion
 
Mutation detailsA concatemer of sleeping beauty transposable elements each consisting of a floxed PGK-neo cassette followed by inverted repeat direct repeat sequence specific for sleeping beauty transposase flanking human BCL2 splice acceptor sequence, IRES, lacZ, poly A signal, a chimeric CAG promoter containing sequence from the chicken ACTB promoter, GFP, and splice donor sequence. Expression of GFP is not seen in the concatemer (in 7 of 8 founders). Transposition inside a gene should result in ubiquitous expression of GFP by the ACTB promoter and expression of lacZ driven by the endogenous promoter. Transposition by sleeping beauty transposase does not move the PGK-neo cassette. (J:104039)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
References
Original:  J:104039 Horie K, et al., Characterization of Sleeping Beauty transposition and its application to genetic screening in mice. Mol Cell Biol. 2003 Dec;23(24):9189-207
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory