Antibody:	anti-Biotinidase
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS17: otocyst mesenchyme	Present	Regionally restricted	1B	Expression was detected in precursor cells in the periotic mesenchyme migrating on both sides of the otocyst.
TS17: acoustic ganglion	Present		1B	Expression was detected in cochlear ganglion (CG) neuron precursors located ventral to the otocyst.
TS17: otocyst epithelium	Present	Regionally restricted	1C , 1D , 1B	Expression was detected in cells and fibers in the otic epithelium facing the neural tube and the dorsal region of the epithelium.
TS17: hindbrain	Present	Regionally restricted	1B	Expression was detected in the subpial region, the floorplate, and the commissural fibers.
TS17: blood vessel	Present		1B	Expression was detected in endothelial cells of blood vessels invading the neural tube.

Structure	Level	Pattern	Image	Note
TS20: vestibulo-cochlear ganglion	Present	Regionally restricted	2B , 2A , 2D , 2C	Expression was detected in the perikarya of the ganglion cell bodies and the bipolar processes peripherally and centrally. Expression was strong in precursor cells migrating from the epithelium across the basal lamina.
TS20: inner ear epithelium	Present	Uniform	2A	Expression was detected throughout the sensory epithelium.
TS20: hindbrain	Present	Regionally restricted	2A	Expression was detected in a ventrolateral piece of the neural tube.
TS20: cochlear duct	Present	Regionally restricted	2A	Expression was detected along the outer membrane of the cochlear duct.

Structure	Level	Pattern	Image	Note
TS20: 4th ventricle lateral recess	Present	Regionally restricted	2E	Expression was detected in proliferating epithelial cells.

Structure	Level	Pattern	Image	Note
TS22: vestibulocochlear nerve cochlear component	Present	Regionally restricted	3B	Expression was detected in fibers from the cochlear nerve.
TS22: cochlear ganglion	Present	Regionally restricted	3B , 3C , 3E , 3D	Expression was detected in the cochlear ganglion and its peripheral processes.
TS22: inner ear epithelium	Present	Regionally restricted	3E , 3B , 3C , 3D	Expression was detected in a strand of cells skirting the edge of the sensory epithelium to reach the abneural side.

Structure	Level	Pattern	Image	Note
TS22: 4th ventricle lateral recess	Present	Regionally restricted	4B , 4A	Expression was detected in precursor cells migrating from the periventricular zone.

Structure	Level	Pattern	Image	Note
TS25: cochlear ganglion	Present
TS25: vestibular ganglion	Present
TS25: inner ear epithelium	Present	Regionally restricted		Expression was detected in the sensory epithelium.
TS25: cochlea	Present	Regionally restricted		Expression was detected in the hair cell layers and was more intense basally.

Structure	Level	Pattern	Image	Note
TS27: organ of Corti	Present	Regionally restricted	5A	Expression was detected in the apical ends of the epithelial cells, including the ciliary tufts. The basal ends of the epithelial cells are less heavily stained.
TS27: cochlear ganglion	Present	Regionally restricted	5B , 5A	Expression was detected in the proximal region of the ganglion, the intraganglionic spiral bundle and the central ganglionic processes. In the neurons, staining was primarily in the perikaryon and the nucleolus.
TS27: vestibular ganglion	Present		5A

Structure	Level	Pattern	Image	Note
TS27: brainstem	Present	Regionally restricted	5C	Expression was detected in the cochlear nucleus.

Structure	Level	Pattern	Image	Note
TS28: organ of Corti	Present	Regionally restricted	6A , 6B	Expression was detected in the bases of the pillar cells bordering the tunnel floor, the radial fibers and was intense in the inner and outer hair cell layers.
TS28: osseus spiral lamina	Present	Regionally restricted	6A	Expression was detected in nerve fibers running between the organ of Corti and the cochlear ganglion.
TS28: cochlear ganglion	Present	Regionally restricted	6A , 6C	Type 1, Type 2 and Schwann cells were stained.

Structure	Level	Pattern	Image	Note
TS28: brainstem	Present	Regionally restricted	7A , 7C , 7D , 7B	Expression was detected in the dorsal and posteroventral cochlear nucleus (CN). In the dorsal CN, small intensely stained cells below the ependymal surface were noted. Some giant and corn cells, and cells of the internal lamella were also stained.

Structure	Level	Pattern	Image	Note
TS28: brainstem	Present	Regionally restricted	8A	Expression was detected in the posteroventral part of the anteroventral part of the cochlear nucleus, and large cells of the dorsal and ventral cochlear nucleus.

Structure	Level	Pattern	Image	Note
TS28: cochlear ganglion	Present	Regionally restricted	8C , 8B	Expression was detected in type 1 cells. Mild staining was seen in type 2 cells.
TS28: vestibulocochlear nerve cochlear component	Absent		8C , 8B	The cochlear nerve fibers were not stained.
TS28: organ of Corti	Present	Regionally restricted	8C	Expression was detected in the hair cell layers.
TS28: spiral limbus	Weak		8C
TS28: tectorial membrane	Weak		8C

Structure	Level	Pattern	Image	Note
TS28: vestibular ganglion	Strong	Regionally restricted	8D	Expression was detected in all three sizes of ganglion cells, but a significant fraction of each cell type is unstained or lightly stained. The perikaryal cytoplasm and nucleolus are stained.
TS28: vestibulocochlear nerve vestibular component	Absent		8D

Structure	Level	Pattern	Image	Note
TS28: cochlear ganglion	Present		9B , 9A	Expression was detected in perikarya of many type 1 and some type 2 cells. Schwann cells surrounding and within the chochlear ganglion are stained.
TS28: osseus spiral lamina	Present	Regionally restricted	9A , 9C	Expression was detected in the fibers running from the cochlear ganglion to the organ of Corti.
TS28: organ of Corti	Present	Regionally restricted	9C , 9C inset , 9A	Expression was detected in the inner and outer spiral bundle, the outer pillar cells, the inner and outer hair cells and the inner radial fibers.

Structure	Level	Pattern	Image	Note
TS28: brainstem	Present		9D inset , 9D
TS28: vestibulocochlear nerve cochlear component	Present	Regionally restricted	9D , 9D inset	Expression was detected on a cochlear nerve fiber endbulb.