Gene Expression Data
Assay Details
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Assay
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Reference:
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J:45489
Zabludoff SD, et al., p27Kip1 is expressed transiently in developing myotomes and enhances myogenesis. Cell Growth Differ. 1998 Jan;9(1):1-11
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3611792
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Gene symbol:
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Cdkn1b
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Gene name:
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cyclin dependent kinase inhibitor 1B
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Antibody:
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Anti-p27
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Detection system:
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Secondary antibody coupled to fluorescein
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Results
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Specimen
not shown:
embryonic day 9.5
(more  )
Specimen
not shown:
(close  )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: trunk myotome |
Present |
Regionally restricted |
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Expression was detected in only a few cells of forelimb level myotomes but no other cells in the paraxial mesoderm.
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| TS15: trunk paraxial mesenchyme |
Present |
Regionally restricted |
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Expression was detected in only a few cells of forelimb level myotomes but no other cells in the paraxial mesoderm.
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Specimen
1A,1D:
embryonic day 10.5
(more )
Note: Double labeled: green - Cdkn1b (p27); red - Myog (assay MGI:3611793).
Specimen
1A,1D:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Cdkn1b (p27); red - Myog (assay MGI:3611793).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: trunk myotome |
Present |
Regionally restricted |
1A
,
1D
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Expression was detected in forelimb level myotomal cells but no other cells in the paraxial mesoderm.
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| TS17: trunk paraxial mesenchyme |
Present |
Regionally restricted |
1A
,
1D
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Expression was detected in forelimb level myotomal cells but no other cells in the paraxial mesoderm.
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Specimen
2A p27:
embryonic day 10.5
(more )
Note: Double labeled: green - Cdkn1b (p27); red - Myod1 (assay MGI:3611794).
Specimen
2A p27:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Cdkn1b (p27); red - Myod1 (assay MGI:3611794).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: trunk myotome |
Present |
Regionally restricted |
2A p27
,
2A MyoD/p27
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Expression was detected in forelimb level myotomal cells but no other cells in the paraxial mesoderm. All cells that were immunopositive were also positive for the expression of MyoD (see Assay MGI:3611794).
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| TS17: trunk paraxial mesenchyme |
Present |
Regionally restricted |
2A p27
,
2A MyoD/p27
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Expression was detected in forelimb level myotomal cells but no other cells in the paraxial mesoderm. All cells that were immunopositive were also positive for the expression of MyoD (see Assay MGI:3611794).
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Specimen
2B p27:
embryonic day 10.5
(more )
Note: Double labeled: green - Cdkn1b (p27); red - Actn2 (assay MGI:3611795) and Actn3 (assay MGI:3611796).
Specimen
2B p27:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Cdkn1b (p27); red - Actn2 (assay MGI:3611795) and Actn3 (assay MGI:3611796).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: trunk myotome |
Present |
Regionally restricted |
2B [alpha]-actinin/p27
,
2B p27
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Expression was detected in forelimb level myotomal cells but no other cells in the paraxial mesoderm.
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| TS17: trunk paraxial mesenchyme |
Present |
Regionally restricted |
2B [alpha]-actinin/p27
,
2B p27
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Expression was detected in forelimb level myotomal cells but no other cells in the paraxial mesoderm.
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Specimen
2C p27 +/-:
embryonic day 10.5; Cdkn1btm1Mlf/Cdkn1b +
(more )
Note: Double labeled: green - Cdkn1b (p27); red - Myog (assay MGI:3611793).
Specimen
2C p27 +/-:
(close )
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Genetic Background:
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involves: 129S4/SvJaeSor
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Mutant Allele(s):
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Cdkn1btm1Mlf/Cdkn1b+
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Cdkn1b (p27); red - Myog (assay MGI:3611793).
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Specimen
2C p27 -/-:
(close )
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Genetic Background:
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involves: 129S4/SvJaeSor
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Mutant Allele(s):
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Cdkn1btm1Mlf/Cdkn1btm1Mlf
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Cdkn1b (p27); red - Myog (assay MGI:3611793).
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Specimen
3 p27 upper:
embryonic day 10.5
(more )
Note: Double labeled: green - Cdkn1b (p27); red - Myog (assay MGI:3611793).
Specimen
3 p27 upper:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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|
Note:
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Double labeled: green - Cdkn1b (p27); red - Myog (assay MGI:3611793).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: trunk myotome |
Present |
Regionally restricted |
3_E10.5 p27
,
3_E10.5 Myogenin/p27
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Expression was detected in forelimb level myotomal cells but no other cells in the paraxial mesoderm. Only a subset of cells that were immunopositive were also positive for the expression of Myogenin (see Assay MGI:3611793).
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| TS17: trunk paraxial mesenchyme |
Present |
Regionally restricted |
3_E10.5 p27
,
3_E10.5 Myogenin/p27
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Expression was detected in forelimb level myotomal cells but no other cells in the paraxial mesoderm. Only a subset of cells that were immunopositive were also positive for the expression of Myogenin (see Assay MGI:3611793).
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Specimen
3 p27 lower:
embryonic day 11.5
(more )
Note: Double labeled: green - Cdkn1b (p27); red - Myog (assay MGI:3611793).
Specimen
3 p27 lower:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Cdkn1b (p27); red - Myog (assay MGI:3611793).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: trunk myotome |
Absent |
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3_E11.5 p27
,
3_E11.5 Myogenin/p27
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| TS19: nervous system |
Strong |
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3_E11.5 p27
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3_E11.5 Myogenin/p27
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| TS19: dorsal root ganglion |
Strong |
Regionally restricted |
3_E11.5 p27
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3_E11.5 Myogenin/p27
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Expression was strong in dorsal root ganglia adjacent to myotome-derived muscle.
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