Gene Expression Data
Assay Details
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Assay
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Reference:
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J:72408
Birkenhager R, et al., Mutation of BSND causes Bartter syndrome with sensorineural deafness and kidney failure. Nat Genet. 2001 Nov;29(3):310-4
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3611688
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Gene symbol:
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Bsnd
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Gene name:
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barttin CLCNK type accessory beta subunit
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Probe:
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Bsnd cDNA
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Probe preparation:
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Antisense RNA
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Results
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Specimen
5A/B/C/D:
postnatal week 6
(more  )
Note: Figs. 5b, 5c and 5d are higher magnification images of Fig. 5a
Specimen
5A/B/C/D:
(close  )
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Genetic Background:
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Not Specified
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Age:
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postnatal week 6
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Figs. 5b, 5c and 5d are higher magnification images of Fig. 5a
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: renal medulla |
Present |
Regionally restricted |
5a
,
5d
,
5b
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Expression is localized in inner and outer stripes in the outer medulla, most probably representing thick ascending limb. In the inner medulla the expression is consistent with it being in the thin ascending limb tubules.
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| TS28: renal cortex |
Present |
Regionally restricted |
5b
,
5c
,
5a
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Expression is present in a subset of renal tubules of the cortex, probably representing distal convoluted tubules or cortical collecting duct. Expression does extend all the way to the tip of the papilla.
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Specimen
embryonic kidney:
embryonic day 18.5
(more )
Specimen
embryonic kidney:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cortical renal tubule |
Present |
Regionally restricted |
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Expression is present in the thin limb of Henle's loop and distal convoluted tubule.
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Specimen
5E:
embryonic day 18.5
(more )
Specimen
5E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cochlear duct |
Strong |
Regionally restricted |
5e
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Expression is present in the marginal cells of the stria vascularis in the cochlea.
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Specimen
5F:
embryonic day 18.5
(more )
Specimen
5F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: inner ear vestibular component |
Strong |
Regionally restricted |
5f
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Expression is localized in dark cells at the base of the crista ampullaris of the vestibular organ.
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Analysis Tools