Probe:	Kcnip2 cDNA1
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	Expression data are summarized in Table 1 (see below) which is located in the supplemental material of the reference.

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Present		4B
TS28: cortical layer III	Present		4B
TS28: cortical layer IV	Present		4B
TS28: cortical layer V	Present		4B
TS28: cortical layer VI	Present		4B
TS28: main olfactory bulb glomerular layer	Present		4B
TS28: main olfactory bulb granule cell layer	Present		4B
TS28: dentate gyrus granule cell layer	Strong	Uniform	4B
TS28: hippocampus CA1	Strong	Uniform	4B
TS28: hippocampus CA2	Strong	Uniform	4B
TS28: hippocampus CA3	Strong	Uniform	4B
TS28: accumbens nucleus	Strong	Regionally restricted	4B	Expression is in most neurons.
TS28: caudate-putamen	Strong	Regionally restricted	4B	Expression is in most neurons.
TS28: pallidum	Absent		4B	Absent in ventral pallidum.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Present		4F
TS28: main olfactory bulb granule cell layer	Present		4F

Structure	Level	Pattern	Image	Note
TS28: piriform cortex	Strong		4J
TS28: accumbens nucleus	Strong	Regionally restricted	4J	Expression is in most neurons.
TS28: caudate-putamen	Strong	Regionally restricted	4J	Expression is in most neurons.
TS28: pallidum	Absent		4J	Absent in ventral pallidum.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus granule cell layer	Strong	Uniform	4N
TS28: hippocampus CA1	Strong	Uniform	4N
TS28: hippocampus CA2	Strong	Uniform	4N
TS28: hippocampus CA3	Strong	Uniform	4N

Structure	Level	Pattern	Image	Note
TS27: spinal cord	Weak		5G
TS27: dorsal root ganglion	Strong		5G

Structure	Level	Pattern	Image	Note
TS28: spinal cord	Absent		5H

Structure	Level	Pattern	Image	Note
TS28: dorsal root ganglion	Moderate	Regionally restricted	5J , 5I	Expression is in a small subpopulation.

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Present		5K , 5L
TS28: cortical layer III	Present		5K , 5L
TS28: cortical layer IV	Present		5K , 5L
TS28: cortical layer V	Present		5K , 5L
TS28: cortical layer VI	Present		5K , 5L

Structure	Level	Pattern	Image	Note
TS28: accumbens nucleus	Strong
TS28: amygdala	Strong
TS28: caudate-putamen	Strong
TS28: dentate gyrus granule cell layer	Strong
TS28: hippocampus CA1	Strong
TS28: hippocampus CA2	Strong
TS28: hippocampus CA3	Strong
TS28: main olfactory bulb glomerular layer	Strong
TS28: main olfactory bulb granule cell layer	Strong
TS28: piriform cortex	Strong
TS28: telencephalon	Strong	Regionally restricted		Expression is strong in the lateral septum and the interstitial nucleus of the posterior limb of the anterior commissure, and is absent in the medial septum.
TS28: anterior olfactory nucleus	Moderate
TS28: cortical layer II	Moderate
TS28: cortical layer III	Moderate
TS28: cortical layer IV	Moderate
TS28: cortical layer V	Moderate
TS28: cortical layer VI	Moderate
TS28: cuneate nucleus	Moderate
TS28: dorsal root ganglion	Moderate
TS28: gigantocellular reticular nucleus	Moderate
TS28: inferior colliculus	Moderate
TS28: reticular thalamic nucleus	Moderate
TS28: mammillary body	Moderate	Regionally restricted		Expression is in the premammillary nucleus and is absent in the mammillary nucleus.
TS28: dorsomedial hypothalamic nucleus	Weak
TS28: lateral habenular nucleus	Weak
TS28: medial habenular nucleus	Weak
TS28: parafascicular nucleus	Weak
TS28: superior colliculus	Weak
TS28: ventromedial hypothalamic nucleus	Weak
TS28: cerebellum Purkinje cell layer	Absent
TS28: cerebellum granule cell layer	Absent
TS28: cerebellum molecular layer	Absent
TS28: globus pallidus	Absent
TS28: main olfactory bulb mitral cell layer	Absent
TS28: medial preoptic nucleus	Absent
TS28: medial vestibular nucleus	Absent
TS28: pontine nucleus	Absent
TS28: spinal cord dorsal horn	Absent
TS28: spinal cord ventral horn	Absent
TS28: pallidum	Absent			Expression is absent in the ventral pallidum.