Probe:	Kcnip1 cDNA1
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	Expression data are summarized in Table 1 (see below) which is located in the supplemental material of the reference.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb granule cell layer	Strong		4A
TS28: hippocampus region	Strong	Single cells	4A	Expression is in scattered neurons around the CA regions.
TS28: accumbens nucleus	Strong	Single cells	4A	Expression is in scattered neurons.
TS28: basal ganglia	Strong	Single cells	4A	Expression is in scattered neurons.
TS28: caudate-putamen	Strong	Single cells	4A	Expression is in scattered neurons.
TS28: dentate gyrus	Strong	Single cells	4A	Expression is in scattered neurons.
TS28: hippocampus molecular layer	Strong	Single cells	4A	Expression is in scattered neurons.
TS28: pallidum	Strong	Single cells	4A	Expression is in scattered neurons.
TS28: cortical layer II	Present		4A
TS28: cortical layer III	Present		4A
TS28: cortical layer IV	Present		4A
TS28: cortical layer V	Present		4A
TS28: cortical layer VI	Present		4A

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb granule cell layer	Strong		4E

Structure	Level	Pattern	Image	Note
TS28: accumbens nucleus	Strong	Single cells	4I	Expression is in scattered neurons.
TS28: basal ganglia	Strong	Single cells	4I	Expression is in scattered neurons.
TS28: caudate-putamen	Strong	Single cells	4I	Expression is in scattered neurons.
TS28: pallidum	Strong	Single cells	4I	Expression is in scattered neurons.
TS28: cortical layer II	Present	Single cells	4I	Expression is in scattered neurons.
TS28: cortical layer III	Present	Single cells	4I	Expression is in scattered neurons.
TS28: cortical layer IV	Present	Single cells	4I	Expression is in scattered neurons.
TS28: cortical layer V	Present	Single cells	4I	Expression is in scattered neurons.
TS28: cortical layer VI	Present	Single cells	4I	Expression is in scattered neurons.
TS28: piriform cortex	Absent		4I

Structure	Level	Pattern	Image	Note
TS28: hippocampus region	Strong	Single cells	4M	Expression is in scattered neurons around the CA regions.
TS28: dentate gyrus	Strong	Single cells	4M	Expression is in scattered neurons.
TS28: hippocampus molecular layer	Strong	Single cells	4M	Expression is in scattered neurons.

Structure	Level	Pattern	Image	Note
TS27: spinal cord	Strong		5A
TS27: dorsal root ganglion	Moderate		5A

Structure	Level	Pattern	Image	Note
TS28: spinal cord	Strong		5B

Structure	Level	Pattern	Image	Note
TS28: dorsal root ganglion	Moderate	Regionally restricted	5C , 5D	Expression is in a small subpopulation.

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Present		5E , 5F
TS28: cortical layer III	Present		5E , 5F
TS28: cortical layer IV	Present		5E , 5F
TS28: cortical layer V	Present		5E , 5F
TS28: cortical layer VI	Present		5E , 5F

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong
TS28: dorsomedial hypothalamic nucleus	Strong
TS28: main olfactory bulb granule cell layer	Strong
TS28: medial habenular nucleus	Strong
TS28: reticular thalamic nucleus	Strong
TS28: spinal cord dorsal horn	Strong
TS28: ventromedial hypothalamic nucleus	Strong
TS28: accumbens nucleus	Strong	Single cells		Expression is in scattered neurons.
TS28: caudate-putamen	Strong	Single cells		Expression is in scattered neurons.
TS28: cortical layer II	Strong	Single cells		Expression is in scattered neurons.
TS28: cortical layer III	Strong	Single cells		Expression is in scattered neurons.
TS28: cortical layer IV	Strong	Single cells		Expression is in scattered neurons.
TS28: cortical layer V	Strong	Single cells		Expression is in scattered neurons.
TS28: cortical layer VI	Strong	Single cells		Expression is in scattered neurons.
TS28: dentate gyrus granule cell layer	Strong	Single cells		Expression is in scattered neurons.
TS28: hippocampus CA1	Strong	Single cells		Expression is in scattered neurons.
TS28: hippocampus CA2	Strong	Single cells		Expression is in scattered neurons.
TS28: hippocampus CA3	Strong	Single cells		Expression is in scattered neurons.
TS28: pallidum	Strong	Single cells		Expression is in scattered neurons in the ventral pallidum.
TS28: cuneate nucleus	Moderate
TS28: dorsal root ganglion	Moderate
TS28: medial preoptic nucleus	Moderate
TS28: parafascicular nucleus	Moderate
TS28: spinal cord ventral horn	Moderate
TS28: telencephalon	Moderate	Regionally restricted		Expression is in the lateral and medial septum and is absent in the interstitial nucleus of the posterior limb of the anterior commissure.
TS28: mammillary body	Moderate	Regionally restricted		Expression is in the premammillary nucleus and is absent in the mammillary nucleus.
TS28: thalamus	Moderate	Regionally restricted		Expression is in the mediodorsal nucleus.
TS28: anterior olfactory nucleus	Weak
TS28: inferior colliculus	Weak
TS28: superior colliculus	Weak
TS28: amygdala	Absent
TS28: cerebellum granule cell layer	Absent
TS28: cerebellum molecular layer	Absent
TS28: gigantocellular reticular nucleus	Absent
TS28: globus pallidus	Absent
TS28: lateral habenular nucleus	Absent
TS28: main olfactory bulb glomerular layer	Absent
TS28: main olfactory bulb mitral cell layer	Absent
TS28: medial vestibular nucleus	Absent
TS28: piriform cortex	Absent
TS28: pontine nucleus	Absent