Probe:	R-cadherin probe
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS20: forebrain	Strong	Regionally restricted	4B , 4C , 4D	Expression was detected in the ventricular zone of the anterior rhinencephalon and the hippocampal region.
TS20: cerebral cortex ventricular layer	Strong	Regionally restricted	4B	Expression was detected in the ventricular zone of the corticostriatal border.
TS20: telencephalon	Strong	Regionally restricted	4B	Expression was detected in the ventricular zone of the amygdala.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Weak		4F	Expression level was decreased compared to wild-type.
TS20: forebrain	Weak	Regionally restricted	4G , 4F , 4H	Expression levels were decreased compared to wild-type in the neuroepithelium of anterior rhinencephalon and the hippocampal region.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex ventricular layer	Present	Regionally restricted	4J	Expression was detected in the ventricular zone of the lateral cortex but ceased at the boundary of the cortex and lateral ganglionic eminence. Boundary does not coincide with the cortico-striatal sulcus but extends over lateromost field of the LGE VZ.
TS20: telencephalon	Present	Regionally restricted	4J	Expression was detected in the basal telencephalon in the presumptive globus pallidus.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex ventricular layer	Present	Regionally restricted	4L	Expression was detected in the ventricular zone of the lateral cortex but ceased at the boundary of the cortex and lateral ganglionic eminence. Boundary does not coincide with the cortico-striatal sulcus but extends over lateromost field of the LGE VZ.
TS20: forebrain	Present	Regionally restricted	4L	Expression was detected in the posterior preoptic area but not in the anterior preoptic area. Expression in the posterior preoptic area included the mantle zone.
TS20: telencephalon	Present	Regionally restricted	4L	Expression was detected in the basal telencephalon in the presumptive globus pallidus.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex ventricular layer	Weak	Regionally restricted	4N	Expression was reduced to background levels compared to wild type at the lateral ganglionic border.
TS20: telencephalon	Present	Regionally restricted	4N	Expression was detected in basal telencephalon as in wild type.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex ventricular layer	Weak	Regionally restricted	4P	Expression was reduced to background levels compared to wild type at the lateral ganglionic border.
TS20: forebrain	Weak	Regionally restricted	4P	Expression was abolished in the posterior preoptic area compared to wild type.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present		5B , 5D , 5C , 5E	Expression was detected throughout the cortical thickness including the cortical plate in rostral, medial and posterior cortex.
TS22: cortical plate	Present		5B , 5D , 5C , 5E	Expression was detected throughout the cortical thickness including the cortical plate in rostral, medial and posterior cortex.
TS22: cerebral cortex marginal layer	Present		5E , 5C , 5B , 5D	Expression was detected in rostral, medial and posterior cortex marginal zone.
TS22: telencephalon	Present	Regionally restricted	5B	Expression was detected in basal telencephalon including the presumptive region of pallidum and a differentiating field in the posterior rhinencephalon.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Weak		5J , 5G , 5I , 5H	Expression was weak in rostral, medial and posterior cortex compared to wild-type.
TS22: cerebral cortex marginal layer	Present		5G , 5H , 5J , 5I	Expression was detected in rostral, medial and posterior cortex marginal zone as in wild-type.
TS22: telencephalon	Present	Regionally restricted	5G	Expression was detected in basal telencephalon including the presumptive region of pallidum and a differentiating field in the posterior rhinencephalon as in wild type. Expression was also detected in a vesicle-like structure in the telencephalic wall.

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	6B	Expression was detected in the ventral thalamus.
TS20: forebrain	Present	Regionally restricted	6B	Expression was detected in the optoeminential zone including the eminentia thalami, supraoptic paraventricular area, anterior hypothalamus and posterior optic area.
TS20: diencephalon	Present	Regionally restricted	6B	Expression was detected in the posterior region of the pretectum, the posterior commissure and the zona limitans intrathalamica.
TS20: hypothalamus	Present	Regionally restricted	6B	Expression was detected in the presumptive hypothalamic cell cord and retrochiasmatic area.

Structure	Level	Pattern	Image	Note
TS22: thalamus	Present	Regionally restricted	6D	Expression was detected in the ventral thalamus.
TS22: forebrain	Present	Regionally restricted	6D	Expression was detected in the optoeminential zone including the eminentia thalami, supraoptic paraventricular area, anterior hypothalamus and posterior optic area.
TS22: hypothalamus	Present	Regionally restricted	6D	Expression was detected in several hypothalamic regions: the suprachiasmatic area, hypothalamic cell cord, the tuberal hypothalamus and the retrochiasmatic domain.

Structure	Level	Pattern	Image	Note
TS20: thalamus	Weak	Regionally restricted	6F	Expression was down-regulated to a level only faintly above the background compared to wild-type.
TS20: hypothalamus	Present	Regionally restricted	6F	Expression was detected in the presumptive hypothalamic cell cord and retrochiasmatic area as in wild type.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex subventricular zone	Present		7E , 7B
TS26: cerebral cortex ventricular zone	Present		7E , 7B
TS26: corpus striatum	Present	Regionally restricted	7B	Expression was detected in a differentiating field in the basal striatum.
TS26: telencephalon	Present	Regionally restricted	7B	Expression was detected in the presumptive pallidum and the region of the amygdala.
TS26: forebrain	Present	Regionally restricted	7B	Expression was detected in the basal rhinencephalon.
TS26: cortical plate	Present	Regionally restricted	7B	In the rostral-most regions (including the insular and perirhinal cortex), the level of expression appears higher.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex subventricular zone	Weak		7G , 7J	Expression was severely decreased compared to wild-type.
TS26: cerebral cortex ventricular zone	Weak		7G , 7J	Expression was severely decreased compared to wild-type.

Structure	Level	Pattern	Image	Note
TS26: main olfactory bulb	Present	Regionally restricted	8B	Expression was detected in the ventricular and subventricular zones, the internal granular layer and the mitral layer.
TS26: cerebral cortex subventricular zone	Present		8B
TS26: cerebral cortex ventricular zone	Present		8B
TS26: cortical plate	Present	Regionally restricted	8B	In the rostral-most regions (including the insular and perirhinal cortex), the level of expression appears higher.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex subventricular zone	Present		8D
TS26: cerebral cortex ventricular zone	Present		8D
TS26: cerebral cortex marginal zone	Strong		8D , 8H
TS26: cerebral cortex	Strong	Regionally restricted	8H , 8D	Expression was strong in a layer of cells that appeared to be below the subplate.
TS26: olfactory cortex marginal layer	Present		8H , 8D

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex subventricular zone	Weak		8L	Expression was severely decreased compared to wild-type.
TS26: cerebral cortex ventricular zone	Weak		8L	Expression was severely decreased compared to wild-type.
TS26: cerebral cortex marginal zone	Strong		8L	Expression was unchanged in the marginal zone compared to wild-type.
TS26: olfactory cortex	Present	Regionally restricted	8L	Expression was detected in the superficial layer as in wild type. Expression was absent from the layer of cells below the subplate.
TS26: telencephalon	Present	Regionally restricted	8L	Expression was detected in a vesicle-like structure.