Probe:	Podxl2 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	After cryosectioning, tissue was fixed for 10' in 4% paraformaldehyde. The author divided the genes analyzed in this publication into 4 groups based on their expression patterns. The expression pattern of this gene was placed in group 4 (See Table 1).

Structure	Level	Pattern	Image	Note
TS20: cerebellum primordium	Present	Regionally restricted	6A , 6B	Expression was detected in the dorsal cells of the cerebellar primordium.
TS20: spinal cord	Present		6A , 6B
TS20: medulla oblongata	Present	Regionally restricted	6B , 6A	Expression was detected in the anterior part of the medulla oblongata but was absent from the neuroepithelium.
TS20: hindbrain	Present	Regionally restricted	6A , 6B	Expression was detected in the neuroepithelium.
TS20: midbrain	Present	Regionally restricted	6B , 6A	Expression was detected in the neuroepithelium.
TS20: central nervous system	Present	Regionally restricted	6A , 6B	Expression was detected in the neuroepithelium of the midbrain and hindbrain, other regions of the central nervous system were void of expression in the neuroepithelium.

Structure	Level	Pattern	Image	Note
TS22: peripheral nervous system	Present		6C
TS22: cranial ganglion	Present		6C
TS22: facial ganglion	Present		6C
TS22: facial nerve	Present		6C

Structure	Level	Pattern	Image	Note
TS22: gut	Present	Regionally restricted	6D	Expression was detected in the gut wall smooth muscle cells.

Structure	Level	Pattern	Image	Note
TS22: olfactory epithelium	Present		6E

Structure	Level	Pattern	Image	Note
TS24: renal cortex	Present		6F

Structure	Level	Pattern	Image	Note
TS26: brain	Present		6G
TS26: olfactory lobe	Present	Regionally restricted	6G	Expression was detected in the olfactory bulb.
TS26: cerebral cortex	Present	Regionally restricted	6G	Expression was detected in some layers.
TS26: cerebellum	Present		6G

Structure	Level	Pattern	Image	Note
TS20: central nervous system	Present
TS20: peripheral nervous system	Present
TS20: retina	Present

Structure	Level	Pattern	Image	Note
TS22: central nervous system	Present
TS22: peripheral nervous system	Present
TS22: renal cortex	Present
TS22: retina	Present
TS22: intestine	Present	Regionally restricted		Expression was detected in smooth muscle cells.
TS22: stomach	Present	Regionally restricted		Expression was detected in smooth muscle cells.
TS22: tongue	Present	Regionally restricted		Expression was detected in smooth muscle cells.
TS22: respiratory tract	Present	Regionally restricted		Expression was detected in smooth muscle cells of bronchi.

Structure	Level	Pattern	Image	Note
TS24: central nervous system	Present
TS24: peripheral nervous system	Present
TS24: retina	Present

Structure	Level	Pattern	Image	Note
TS26: central nervous system	Present
TS26: peripheral nervous system	Present
TS26: retina	Present