Gene Expression Data
Assay Details
Assay
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Reference:
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J:99372
van der Zwaag B, et al., Identifying new candidate genes for hereditary facial paresis on chromosome 3q21-q22 by RNA in situ hybridization in mouse. Genomics. 2005 Jul;86(1):55-67
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3605532
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Gene symbol:
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Chst13
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Gene name:
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carbohydrate sulfotransferase 13
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Probe:
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Chst13 cDNA1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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After cryosectioning, tissue was fixed for 10' in 4% paraformaldehyde. The author divided the genes analyzed in this publication into 4 groups based on their expression patterns. The expression pattern of this gene was placed in group 3 (See Table 1).
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Results
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Specimen
2A:
embryonic day 14.5
(more )
Note: The specimen was the lower half of the embryo.
Specimen
2A:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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The specimen was the lower half of the embryo.
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Structure |
Level |
Pattern |
Image |
Note |
TS22: metanephros |
Present |
Regionally restricted |
2A
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Expression was detected in clusters of cells.
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Specimen
2B:
embryonic day 14.5
(more )
Note: The specimen was the head.
Specimen
2B:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 14.5
|
Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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The specimen was the head.
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Specimen
not shown:
embryonic day 12.5
(more )
Specimen
not shown:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Specimen
not shown:
embryonic day 18.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 18.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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