Summary | Transgene origin | Transgene description | Expression | Phenotypes | Disease models | Find Mice (IMSR) | Notes | References

Symbol:	Tg(Scgb1a1-Scnn1b)6608Bouc
Name:	transgene insertion 6608, Richard Boucher
MGI ID:	MGI:3605471
Synonyms:	betaENaC-Tg
Transgene:	Tg(Scgb1a1-Scnn1b)6608Bouc  Location: unknown  Genetic Position: Chr5, Syntenic
Alliance:	Tg(Scgb1a1-Scnn1b)6608Bouc page

Strain of Origin:

(C3H x C57BL/6)F1

Transgene Type:    Transgenic (Inserted expressed sequence) Mutation:    Insertion   Tg(Scgb1a1-Scnn1b)6608Bouc expresses 1 gene Transgene expresses: Organism Expressed Gene Note mouse Scnn1b (MGI:104696)   Mutation details: Approximately 2330 base pairs of 5' flanking sequence from the rat airway-specific Scgb1a1 (also called the Clara cell secretory protein) gene were inserted into the pTG1 vector upstream of exon 1 (104 base pairs, including the transcription initiation site), intron 1 (947 base pairs) and 10 base pairs of exon 2 from the mouse transthyretin gene; the protein-coding region of the mouse Scnn1b cDNA, inserted just downstream of the transthyretin exon 2 splice acceptor, is followed by the SV40 early-region polyadenylation signal. In situ hybridization with a transgene-specific antisense probe confirmed expression in airway epithelia of the lung. The insertion site was identified by in situ hybridization; it is located on chromosome 5 near the B-C1 chromosome band junction. (J:91139, J:101822, J:101823, J:220663)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

5 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 5 strains available      Cell Lines: 0 lines available

Original:	J:91139 Mall M, et al., Increased airway epithelial Na+ absorption produces cystic fibrosis-like lung disease in mice. Nat Med. 2004 May;10(5):487-93
All:	45 reference(s)