Dnmt3a RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:100438 Chen HW, et al., Transcriptome analysis in blastocyst hatching by cDNA microarray. Hum Reprod. 2005 Sep;20(9):2492-501
Assay type:	RT-PCR
MGI Accession ID:	MGI:3603602
Gene symbol:	Dnmt3a
Gene name:	DNA methyltransferase 3A

Probe:	Dnmt3a-pC, Dnmt3a-pD
Probe preparation:	labelled with SYBR green
Assay notes:	These data were obtained using real-time quantitative RT-PCR.

Results

Image: 2B Dnmt3a

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified (b)	Amount	Genetic Background	Mutant Allele(s)	Sex
2-cell	E1.0 (a)	TS2: 2-cell stage conceptus	Strong (c)	Not Specified; total RNA	ICR		Not Specified
8-cell	E2.5 (a)	TS3: 8-cell stage conceptus	Present	Not Specified; total RNA	ICR		Not Specified
Morula	E3.0 (a)	TS3: morula-stage conceptus	Present	Not Specified; total RNA	ICR		Not Specified
Pre-hatch Blastocyst	E3.5 (a)	TS4: blastocyst-stage conceptus	Present	Not Specified; total RNA	ICR		Not Specified
Hatch Blastocyst	E4.0 (a)	TS5: blastocyst-stage conceptus	Present	Not Specified; total RNA	ICR		Not Specified

Notes:
(a) Age assigned by curator based on morphological criteria supplied by authors.
(b) Expression levels are reported as relative to the levels observed in pre-hatching blastocysts.
(c) Expression levels are sharply downregulated after the 2-cell stage.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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