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Gene Expression Data
Assay Details
Assay
Reference: J:100438 Chen HW, et al., Transcriptome analysis in blastocyst hatching by cDNA microarray. Hum Reprod. 2005 Sep;20(9):2492-501
Assay type: RT-PCR
MGI Accession ID: MGI:3603599
Gene symbol: Dnmt1
Gene name: DNA methyltransferase 1
Probe: Dnmt1-pC, Dnmt1-pD
Probe preparation: labelled with SYBR green
Assay notes: These data were obtained using real-time quantitative RT-PCR. Authors detailed two sets of primers for this gene but did not specify which was used in this assay; it is possible another set (MGI:3603580) was used instead.
Results Image: 2B Dnmt1
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (b) Amount Genetic BackgroundMutant Allele(s)Sex
2-cell E1.0 (a) TS2: 2-cell stage conceptus Strong Not Specified; total RNA ICR Not Specified
8-cell E2.5 (a) TS3: 8-cell stage conceptus Present (c) Not Specified; total RNA ICR Not Specified
Morula E3.0 (a) TS3: morula-stage conceptus Present (c) Not Specified; total RNA ICR Not Specified
Pre-hatch Blastocyst E3.5 (a) TS4: blastocyst-stage conceptus Weak Not Specified; total RNA ICR Not Specified
Hatch Blastocyst E4.0 (a) TS5: blastocyst-stage conceptus Present (d) Not Specified; total RNA ICR Not Specified
Notes:
(a) Age assigned by curator based on morphological criteria supplied by authors.
(b) Expression levels are reported as relative to the levels observed in pre-hatching blastocysts.
(c) Expression levels are reduced compared to those observed in the 2-cell embryo.
(d) Expression levels are significantly higher than those seen in pre-hatched blastocysts.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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