Probe:	pKLS4
Probe preparation:	Antisense labelled with P33 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS15: heart	Present		4A , 4B
TS15: nervous system	Present		4A , 4B
TS15: extraembryonic component	Absent		4A , 4B	There is no expression in extra embryonic membranes, including yolk sac and Reiherdt's membranes.
TS15: yolk sac	Absent		4A , 4B	There is no expression in extra embryonic membranes, including yolk sac and Reiherdt's membranes.
TS28: decidua	Present		4A , 4B	Expression is present in the decidua capsularis.
TS28: placenta	Present	Regionally restricted	4A , 4B	Expression is present in the basalis of the placenta.

Structure	Level	Pattern	Image	Note
TS28: uterus	Absent		4A , 4B

Structure	Level	Pattern	Image	Note
TS24: embryo	Present		4D , 4C	Expression is present in most developing organs.
TS24: forebrain	Strong	Regionally restricted	4D , 4C	Expression levels are high in the hippocampus.
TS24: cerebellum	Strong		4C , 4D
TS24: dorsal root ganglion	Strong		4C , 4D
TS24: spinal cord	Strong		4C , 4D
TS24: trigeminal ganglion	Strong		4C , 4D
TS24: cranial ganglion	Strong		4D , 4C	Expression levels are high in the superior cranial ganglia.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex	Present	Non-Uniform	4F , 4E	Expression is high in the cortex but lower in ventricular layer.
TS25: diencephalon	Present		4F , 4E	Expression levels are lower in the diencephalon than those observed in the cortex.
TS25: ethmoid bone	Present		4F , 4E
TS25: heart	Present		4F , 4E
TS25: intestine	Present		4F , 4E
TS25: liver	Present		4F , 4E
TS25: lung mesenchyme	Present		4F , 4E
TS25: seminal vesicle	Present		4F , 4E
TS25: submandibular gland	Present		4F , 4E
TS25: thymus	Present		4F , 4E
TS25: tongue	Present		4F , 4E
TS25: tooth	Present		4F , 4E
TS25: skeletal muscle	Present	Regionally restricted	4F , 4E	Expression was present in the neck muscle.

Structure	Level	Pattern	Image	Note
TS27: brain	Present		5A , 5B	This is one of the tissues with the highest level of expression.
TS27: cerebellum	Present		5A , 5B	This is one of the tissues with the highest level of expression.
TS27: dorsal root ganglion	Present		5A , 5B	This is one of the tissues with the highest level of expression.
TS27: spinal cord	Present		5A , 5B	This is one of the tissues with the highest level of expression.
TS27: thymus cortex	Present		5A , 5B	This is one of the tissues with the highest level of expression.
TS27: tooth	Present	Regionally restricted	5A , 5B	Expression is present in the ameloblast and odontoblast layers.
TS27: nose	Present		5B , 5A	Expression is present in nasal areas.
TS27: heart	Present		5B , 5A	This is one of the tissues with a lower level of expression.
TS27: liver	Present		5B , 5A	This is one of the tissues with a lower level of expression.
TS27: small intestine	Present		5B , 5A	This is one of the tissues with a lower level of expression.
TS27: submandibular gland	Present		5B , 5A	This is one of the tissues with a lower level of expression.
TS27: tongue	Present		5B , 5A	This is one of the tissues with a lower level of expression.
TS27: aorta	Present	Regionally restricted	5B , 5A	Expression is present in the aorta walls.
TS27: lung connective tissue	Present		5A , 5B	Expression is present in the lung mesenchyme.
TS27: bladder	Absent		5A , 5B	No significant levels of expression were seen.
TS27: colon	Absent		5A , 5B	No significant levels of expression were seen.
TS27: lung epithelium	Absent		5A , 5B	No significant expression was seen in the epithelial bronchioles of the lung.

Structure	Level	Pattern	Image	Note
TS27: thymus cortex	Present		5D

Structure	Level	Pattern	Image	Note
TS27: dorsal root ganglion	Present		5E

Structure	Level	Pattern	Image	Note
TS27: small intestine	Present		5F