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Gene Expression Data
Assay Details
Assay
Reference: J:83680 Chambers I, et al., Functional expression cloning of nanog, a pluripotency sustaining factor in embryonic stem cells. Cell. 2003 May 30;113(5):643-55
Assay type: RNA in situ
MGI Accession ID: MGI:3590318
Gene symbol: Nanog
Gene name: Nanog homeobox
Probe: Nanog cDNA1
Probe preparation: Antisense labelled with digoxigenin RNA
Results
Specimen 4A one-cell embryo: embryonic day 0.5 (more )
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Absent 4A top left
Specimen 4A two-cell embryo: embryonic day 1.0 (more )
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Absent 4A top center
Specimen 4A six-cell embryo: embryonic day 2.0 (more )
Structure Level Pattern Image Note
TS3: 4-8 cell stage embryo Absent 4A top right
Specimen 4A eight-cell embryo: embryonic day 2.5 (more )
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Absent 4A middle left
Specimen 4A late morula: embryonic day 3.0 (more )
Structure Level Pattern Image Note
TS4: compacted morula Present Regionally restricted 4A middle center Expression is present in the interior cells, the future inner cell mass.
Specimen 4A early blastocyst: embryonic day 3.5 (more )
Structure Level Pattern Image Note
TS4: inner cell mass Present 4A middle right
TS4: trophectoderm Absent 4A middle right
Specimen 4A expanded blastocyst: embryonic day 3.75 (more )
Structure Level Pattern Image Note
TS5: inner cell mass Present Regionally restricted 4A lower left Expression is restricted to the epiblast and excluded from the primitive endoderm.
Specimen 4A hatched blastocyst: embryonic day 4.0 (more )
Structure Level Pattern Image Note
TS5: inner cell mass Present Regionally restricted 4A lower center Expression is restricted to the epiblast and excluded from the primitive endoderm.
Specimen 4A implanting blastocyst: embryonic day 4.5 (more )
Structure Level Pattern Image Note
TS6: embryo Absent 4A lower right Expression is lost around the time of implantation.
Specimen 4B female genital ridge: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: primordial germ cell of gonad Present 4B top
Specimen 4B male genital ridge: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: primordial germ cell of gonad Present 4B bottom
Specimen not shown: embryonic day 4.0; Pou5f1tm1Cgre/Pou5f1tm1Cgre (more )
Structure Level Pattern Image Note
TS5: inner cell mass Present Expression is indistinguishable from that seen in wild type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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01/20/2026
MGI 6.24 		The Jackson Laboratory